化学
色谱法
甲酸
肽
质谱法
串联质谱法
酪蛋白
胰蛋白酶
氨基酸
食品科学
生物化学
酶
作者
Qin Chen,Shiyun Lai,Lezhen Dong,Yahui Liu,Daodong Pan,Zhen Wu,Zufang Wu,Ying Zhou,Yiping Ren,Jingshun Zhang,Lingyi Liu,Lianliang Liu
出处
期刊:Food Chemistry
[Elsevier]
日期:2023-08-01
卷期号:430: 137049-137049
被引量:7
标识
DOI:10.1016/j.foodchem.2023.137049
摘要
The ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS) method was built to quantify the casein glycomacropeptide (CGMP) in bovine dairy products accurately based on targeted proteomics. Qualitative analysis of theoretical peptides was carried out using high-resolution mass spectrometry (HRMS) and protein software. Isotope-labeled characteristic peptides were acquired via the labeled amino acid condensation method to correct the matrix effects. Peptide MAIPPK was the representative characteristic peptide for distinguishing the CGMP from κ-casein through trypsin digestion. After optimizing the pre-treatment conditions, the final 8% oxidant concentration was selected and the 10% formic acid concentration with 2.5 h oxidation time. Moreover, the results of methodological verification showed that the recovery rate was 103.7%, meanwhile the precision of inter-day and intra-day was less than 5%. In conclusion, the research demonstrated the characteristic peptide MAIPPK could quantitatively applied to detect CGMP in dairy products.
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