工作流程
微流控
转导(生物物理学)
细胞生物学
转染
细胞
生物
纳米技术
计算机科学
材料科学
基因
遗传学
生物物理学
数据库
作者
Tong Yu,Navdeep Jhita,Peter G. Shankles,Andrew Fedano,Noah B. Kramer,Sunil S. Raikar,Todd Sulchek
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:23 (22): 4804-4820
摘要
We developed a workflow to produce potent gene-edited CAR T cells that combines microfluidic transfection and lentiviral transduction using healthy donor- and patient-derived T cells. We developed an understanding on how biomechanics of cells impact mechanoporation.
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