抗生素
胞外聚合物
抗生素耐药性
电解
废水
细胞外
化学
微生物学
细菌
微生物电解槽
分布(数学)
生物
生物化学
环境工程
生物膜
电极
环境科学
遗传学
数学
电解质
数学分析
物理化学
作者
Shiguang Liu,Zengshuai Zhang,Peng Gu,Kunlun Yang,Yifan Jia,Hengfeng Miao
出处
期刊:Chemosphere
[Elsevier]
日期:2024-09-01
卷期号:364: 143284-143284
标识
DOI:10.1016/j.chemosphere.2024.143284
摘要
Microbial electrolysis cells (MEC) have emerged as a prominent technology for the treatment of antibiotics-containing wastewater in recent years. However, there remains a dearth of comprehensive exploration regarding the influence of extracellular polymers substances (EPS) on the distribution and transmission of antibiotic resistance genes (ARGs) in MEC. In this study, we quantified the distribution of ARGs in MEC by Fluorescence quantitative polymerase chain reaction and explored with emphasis on impact of EPS component on ARGs transmission at under different concentrations of roxithromycin. Results showed that the absolute abundance of ARGs in the electrode biofilm was 1 - 2 orders of magnitude higher than that in the anolyte. Specifically, EPS-associated ARGs accounted for 2.31% - 11.18% of ARGs in electrode biofilm. The presence of elevated roxithromycin concentration led to electroactive microorganisms (Geobacter and Geothrix) as potential hosts of ARGs. In addition, both protein and polysaccharide content in the electrode biofilm increased with increasing roxithromycin concentration and showed positive correlations with EPS-associated ARGs. Fluorescence quenching experiments further elucidated that tryptophan and tyrosine residues in EPS could bind to ARGs effectively, contributing the hindering the ARGs transmission between hosts. Therefore, increased EPS content within electrode biofilm could reduce the concentration of ARGs present in anolyte while also influencing ARGs distribution throughout MEC. This study provides valuable insights into the distribution of ARGs in MEC systems and the role of EPS in regulating ARGs migration.
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