牙龈卟啉单胞菌
生物
转录因子
突变体
微生物学
抄写(语言学)
分子生物学
基因
生物化学
遗传学
细菌
语言学
哲学
作者
Yang Qiu,Xuelian Tan,Zhouyue Lei,Xuan Chen,Jiamin Chen,Tao Gong,Yajie Wu,Yuqing Li,Dingming Huang
摘要
Abstract Porphyromonas gingivalis is a keystone pathogen for periodontitis. The function of the GntR family transcription factor is poorly studied in P. gingivalis . Numerous processes govern bacterial growth. The survival and pathogenicity of P. gingivalis depend heavily on its capacity to acquire amino acids as nutritional sources. In this investigation, a GntR transcription factor, pg1007 , was identified in P. gingivalis , the deletion of which significantly inhibited bacterial growth. The mutant strain also exhibited an increased extracellular activity of gingipains and acylpeptidyl oligopeptidase (AOP). Global gene expression profiling revealed that the expression levels of 59 genes were significantly altered in the Δpg1007 mutant, with an upregulation in gene expression for AOP, ABC transporters, and some membrane proteins. In addition, His‐PG1007 protein was purified as a recombinant protein from Escherichia coli , and the conserved DNA sequence bound by it was determined using electrophoretic mobility shift assays and DNase I footprinting assays. Consequently, this study demonstrated that pg1007 is a crucial transcription factor in P. gingivalis and regulates the bacterial growth and activity of gingipains and AOP. These findings may enhance our understanding of the regulation of bacterial proliferation and protease activity in P. gingivalis .
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