清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Quercetin 3-O-glucuronide-rich lotus leaf extract promotes a Brown-fat-phenotype in C3H10T1/2 mesenchymal stem cells

安普克 产热 线粒体生物发生 化学 槲皮素 PRDM16 产热素 间充质干细胞 细胞生物学 生物化学 褐色脂肪组织 生物 脂肪组织 磷酸化 线粒体 蛋白激酶A 抗氧化剂
作者
Zhenyu Wang,Chaoyi Xue,Xuan Wang,Maomao Zeng,Zhaojun Wang,Qiuming Chen,Jie Chen,Mark Christian,Zhiyong He
出处
期刊:Food Research International [Elsevier BV]
卷期号:163: 112198-112198 被引量:9
标识
DOI:10.1016/j.foodres.2022.112198
摘要

Lotus (Nelumbo nucifera Gaertn.) is an aquatic perennial crop planted worldwide and its leaf (also called "He-Ye") has therapeutic effects on obesity. However, whether the underlying mechanism leads to increased energy expenditure by activation of brown adipocytes has not been clarified. Here, murine C3H10T1/2 mesenchymal stem cells (MSCs) were employed to investigate the effects of ethanol extracts from lotus leaf (LLE) on brown adipocytes formation and the underlying molecular mechanisms. The results showed LLE was rich in polyphenols (383.7 mg/g) and flavonoids (178.3 mg/g), with quercetin 3-O-glucuronide (Q3G) the most abundant (128.2 μg/mg). In LLE-treated C3H10T1/2 MSCs, the expressions of lipolytic factors (e.g., ATGL, HSL, and ABHD5) and brown regulators (e.g., Sirt1, PGC-1α, Cidea, and UCP1) were significantly upregulated compared to that in the untreated MSCs. Furthermore, LLE promoted mitochondrial biogenesis and fatty acid β-oxidation, as evidenced by increases in the expression of Tfam, Cox7A, CoxIV, Cox2, Pparα, and Adrb3. Likewise, enhanced browning and mitochondrial biogenesis were also observed in Q3G-stimulated cells. Importantly, LLE and Q3G induced phosphorylation of AMPK accompanied by a remarkable increase in the brown fat marker UCP1, while pretreatment with Compound C (an AMPK inhibitor) reversed these changes. Moreover, stimulating LLE or Q3G-treated cells with CL316243 (a beta3-AR agonist) increased p-AMPKα/AMPKα ratio and UCP1 protein expression, indicating β3-AR/AMPK signaling may involve in this process. Collectively, these observations suggested that LLE, especially the component Q3G, stimulates thermogenesis by activating brown adipocytes, which may involve the β3-AR/AMPK signaling pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
7秒前
Able完成签到,获得积分10
8秒前
我是笨蛋完成签到 ,获得积分10
11秒前
ceeray23发布了新的文献求助20
13秒前
Owen应助董可以采纳,获得10
25秒前
酷酷妙梦完成签到,获得积分10
30秒前
科研通AI2S应助彦嘉采纳,获得10
53秒前
小二郎应助科研通管家采纳,获得10
1分钟前
hhh2018687完成签到,获得积分10
1分钟前
科研通AI2S应助ceeray23采纳,获得20
1分钟前
忘忧Aquarius完成签到,获得积分10
1分钟前
wujiwuhui完成签到 ,获得积分10
1分钟前
Lny发布了新的文献求助30
2分钟前
sh1ro完成签到,获得积分10
2分钟前
luang应助ceeray23采纳,获得40
2分钟前
2分钟前
ww完成签到,获得积分10
2分钟前
斯文败类应助ceeray23采纳,获得20
2分钟前
机智秋莲发布了新的文献求助20
2分钟前
ChatGPT完成签到,获得积分10
3分钟前
所所应助科研通管家采纳,获得10
3分钟前
3分钟前
3分钟前
ceeray23发布了新的文献求助20
3分钟前
海阔天空完成签到 ,获得积分10
3分钟前
zys发布了新的文献求助10
4分钟前
ffdhdh应助LYZSh采纳,获得10
4分钟前
4分钟前
机智秋莲完成签到,获得积分20
4分钟前
欣欣子完成签到 ,获得积分10
4分钟前
apt完成签到 ,获得积分10
5分钟前
科研通AI2S应助科研通管家采纳,获得10
5分钟前
6分钟前
董可以发布了新的文献求助10
6分钟前
Orange应助董可以采纳,获得10
6分钟前
飞翔的企鹅完成签到,获得积分10
6分钟前
6分钟前
董可以发布了新的文献求助10
6分钟前
LYZSh发布了新的文献求助10
7分钟前
彭于晏应助董可以采纳,获得10
7分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990543
求助须知:如何正确求助?哪些是违规求助? 3532220
关于积分的说明 11256532
捐赠科研通 3271057
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234