Pharmacological nNOS inhibition modified small conductance Ca2+-activated K+ channel without altering Ca2+ dynamics.

阿帕明 SK通道 化学 粘菌毒素 内科学 生物物理学 钾通道 一氧化氮合酶 一氧化氮 钙激活钾通道 神经传导速度 内分泌学
作者
Taro Koya,Masaya Watanabe,Hiroyuki Natsui,Takahide Kadosaka,Takuya Koizumi,Motoki Nakao,Hikaru Hagiwara,Rui Kamada,Taro Temma,Toshihisa Anzai
出处
期刊:American Journal of Physiology-heart and Circulatory Physiology [American Physical Society]
标识
DOI:10.1152/ajpheart.00252.2022
摘要

Atrial fibrillation (AF) is associated with electrical remodeling processes that promote a substrate for the maintenance of AF. Although the small-conductance Ca2+-activated K+ (SK) channel is a key factor in atrial electrical remodeling, the mechanism of its activation remains unclear. Regional nitric oxide (NO) production by neuronal nitric oxide synthase (nNOS) is involved in atrial electrical remodeling. In this study, atrial tachyarrhythmia (ATA) induction and optical mapping were performed on perfused rat hearts. nNOS is pharmacologically inhibited by S-methylthiocitrulline (SMTC). The influence of the SK channel was examined using a specific channel inhibitor, apamin. Parameters such as action potential duration (APD), conduction velocity, and calcium transient (CaT) were evaluated using voltage and calcium optical mapping. The dominant frequency was examined in the analysis of AF dynamics. SMTC (100 nM) increased the inducibility of ATA and apamin (100 nM) mitigated nNOS inhibition-induced arrhythmogenicity. SMTC caused abbreviations and enhanced the spatial dispersion of APD, which was reversed by apamin. By contrast, conduction velocity and other parameters associated with CaT were not affected by SMTC or apamin administration. Apamin reduced the frequency of SMTC-induced ATA. In summary, nNOS inhibition abbreviates APD by modifying the SK channels. A specific SK channel blocker, apamin, mitigated APD abbreviation without alteration of CaT, implying an underlying mechanism of post-translational modification of SK channels.
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