细胞生物学
细胞
生物
电池类型
细胞生长
免疫系统
提吉特
细胞分化
免疫学
T细胞
基因
遗传学
作者
Bo-Hua Gao,Yan Wang,Ye Zhang,Zhen Chen,Guang-Fu Ming
出处
期刊:Folia Biologica
日期:2024-01-01
卷期号:70 (3): 166-178
标识
DOI:10.14712/fb2024070030166
摘要
We aimed to explore the development and cell communication of osteoblasts and osteoclasts with aneuploidy variation in giant cell tumour of bone (GCTB). We predicted the diploid and aneuploid cells in tissue samples using the CopyKAT package. The Monocle2 package was used to analyse differentiation trajectories of aneuploid cells. We used the CellChat package to observe the signalling pathways and ligand-receptor pairs for the two interaction types, “Cell-Cell Contact” and “Secreted Signalling”, respectively. A total of 9,117 cells were obtained including eight cell types. Most aneuploid cells were osteoblasts. As the cell differentiation trajectory matured, we found that aneuploid osteoblasts first increased the inflammatory response activity and then enhanced the ability to activate T cells, whereas osteoclasts gradually enhanced the cellular energy metabolism, cell adhesion, cell proliferation and immune response; the activated biological functions were gradually weakened. The analysis by CellChat indicated that CTLA4 or TIGIT might act as important immune checkpoint genes to attenuate the inhibitory effect of aneuploid osteoclasts on NK/T cells, thereby enhancing the activity of NK/T cells. Our study found that both osteoblasts and osteoclasts might be involved in the development of GCTB, which may provide a new direction for the treatment of GCTB.
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