脚手架
3D生物打印
生物医学工程
颅骨成形术
再生(生物学)
间充质干细胞
材料科学
骨愈合
医学
组织工程
解剖
颅骨
病理
细胞生物学
生物
作者
Yu Huan,Dezhi Zhou,Xiuquan Wu,Xin He,Hongqing Chen,Sanzhong Li,Bo Jia,Yanan Dou,Xiaowei Fei,Shuang Wu,Jialiang Wei,Zhou Fei,Tao Xu,Fei Fei
出处
期刊:Biofabrication
[IOP Publishing]
日期:2023-02-22
卷期号:15 (2): 025016-025016
被引量:17
标识
DOI:10.1088/1758-5090/acbe21
摘要
Although autologous bone (AB) grafting is considered to be the gold standard for cranioplasty, unresolved problems remain, such as surgical-site infections and bone flap absorption. In this study, an AB scaffold was constructed via three-dimensional (3D) bedside-bioprinting technology and used for cranioplasty. To simulate the skull structure, a polycaprolactone shell was designed as an external lamina, and 3D-printed AB and a bone marrow-derived mesenchymal stem cell (BMSC) hydrogel was used to mimic cancellous bone for bone regeneration. Ourin vitroresults showed that the scaffold exhibited excellent cellular affinity and promoted osteogenic differentiation of BMSCs in both two-dimensional and 3D culture systems. The scaffold was implanted in beagle dog cranial defects for up to 9 months, and the scaffold promoted new bone and osteoid formation. Furtherin vivostudies indicated that transplanted BMSCs differentiated into vascular endothelium, cartilage, and bone tissues, whereas native BMSCs were recruited into the defect. The results of this study provide a method for bedside bioprinting of a cranioplasty scaffold for bone regeneration, which opens up another window for clinical applications of 3D printing in the future.
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