异种移植
低温保存
胎儿
类有机物
肾
生物
男科
小型猪
医学
生理学
移植
内科学
怀孕
胚胎
外科
内分泌学
细胞生物学
遗传学
作者
Yuka Inage,Koki Fujimori,Masaki Takasu,Kenji Matsui,Yoshitaka Kinoshita,Keita Morimoto,Nagisa Koda,Shutaro Yamamoto,Kentaro Shimada,Takashi Yokoo,Eiji Kobayashi
摘要
Fetal organs and organoids are important tools for studying organ development. Recently, porcine organs have garnered attention as potential organs for xenotransplantation because of their high degree of similarity to human organs. However, to meet the prompt demand for porcine fetal organs by patients and researchers, effective methods for producing, retrieving, and cryopreserving pig fetuses are indispensable. Therefore, in this study, to collect fetuses for kidney extraction, we employed cesarean sections to preserve the survival and fertility of the mother pig and a method for storing fetal kidneys by long-term cryopreservation. Subsequently, we evaluated the utility of these two methods. We confirmed that the kidneys of pig fetuses retrieved by cesarean section that were cryopreserved for an extended period could resume renal growth when grafted into mice and were capable of forming renal organoids. These results demonstrate the usefulness of long-term cryopreserved fetal pig organs and strongly suggest the effectiveness of our comprehensive system of pig fetus retrieval and fetal organ preservation, thereby highlighting its potential as an accelerator of xenotransplantation research and clinical innovation.
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