Synthesis of nanocomposite hydrogel based carboxymethyl starch/polyvinyl alcohol/nanosilver for biomedical materials

Treatment of infections using wound dressing integrated with multiple functions such as antibacterial activity, non-toxicity, and good mechanical properties has attracted much attention. In this study, carboxymethyl starch/polyvinyl alcohol/citric acid (CMS/PVA/CA) hydrogels containing silver nanoparticles (AgNPs) were prepared. The CMS, PVA and CA were used as polymer matrix and bio-based reducing agents for green synthesis of AgNPs. Silver nitrate (AgNO3) concentrations of 50, 100, and 150 mM were used to obtain nanocomposite hydrogels containing different AgNPs concentrations (AgNPs-50, AgNPs-100 and AgNPs-150, respectively). The minimum inhibitory concentration against E. coli and S. aureus was observed in CMS/PVA/CA hydrogels containing AgNPs-50. Uniform dispersion of AgNPs-100 in the hydrogel provided the highest storage modulus at 56.4 kPa. AgNPs-loaded hydrogels showed low toxicity to human fibroblast cells indicating good biocompatibility. Incorporation of AgNPs demonstrated an enhancement in antibacterial properties and overall mechanical properties, which makes these nanocomposite hydrogels attractive as novel wound dressing materials.