Epidural electrical stimulation effectively restores locomotion function in rats with complete spinal cord injury

脊髓 脊髓损伤 刺激 医学 功能性电刺激 神经假体 硬膜外腔 植入 神经科学 解剖 生物医学工程 麻醉 外科 生物 物理医学与康复 内科学 精神科
作者
Xinran Ji,Peifu Tang,Song Wang,Licheng Zhang,Haitao Fu,Junhao Deng,Gaoxiang Xu,Jia Li
出处
期刊:Neural Regeneration Research [Medknow Publications]
卷期号:16 (3): 573-573 被引量:15
标识
DOI:10.4103/1673-5374.290905
摘要

Epidural electrical stimulation can restore limb motor function after spinal cord injury by reactivating the surviving neural circuits. In previous epidural electrical stimulation studies, single electrode sites and continuous tetanic stimulation have often been used. With this stimulation, the body is prone to declines in tolerance and locomotion coordination. In the present study, rat models of complete spinal cord injury were established by vertically cutting the spinal cord at the T8 level to eliminate disturbance from residual nerve fibers, and were then subjected to epidural electrical stimulation. The flexible extradural electrode had good anatomical topology and matched the shape of the spinal canal of the implanted segment. Simultaneously, the electrode stimulation site was able to be accurately applied to the L2–3 and S1 segments of the spinal cord. To evaluate the biocompatibility of the implanted epidural electrical stimulation electrodes, GFAP/Iba-1 double-labeled immunofluorescence staining was performed on the spinal cord below the electrodes at 7 days after the electrode implantation. Immunofluorescence results revealed no significant differences in the numbers or morphologies of microglia and astrocytes in the spinal cord after electrode implantation, and there was no activated Iba-1+ cell aggregation, indicating that the implant did not cause an inflammatory response in the spinal cord. Rat gait analysis showed that, at 3 days after surgery, gait became coordinated in rats with spinal cord injury under burst stimulation. The regained locomotion could clearly distinguish the support phase and the swing phase and dynamically adjust with the frequency of stimulus distribution. To evaluate the matching degree between the flexible epidural electrode (including three stimulation contacts), vertebral morphology, and the level of the epidural site of the stimulation electrode, micro-CT was used to scan the thoracolumbar vertebrae of rats before and after electrode implantation. Based on the experimental results of gait recovery using three-site stimulation electrodes at L2–3 and S1 combined with burst stimulation in a rat model of spinal cord injury, epidural electrical stimulation is a promising protocol that needs to be further explored. This study was approved by the Animal Ethics Committee of Chinese PLA General Hospital (approval No. 2019-X15-39) on April 19, 2019.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
北枳发布了新的文献求助10
刚刚
美满的冬卉完成签到 ,获得积分10
1秒前
崔雨旋完成签到,获得积分10
1秒前
hhh完成签到,获得积分10
1秒前
sysi发布了新的文献求助10
2秒前
流白发布了新的文献求助10
2秒前
callmecjh完成签到,获得积分10
2秒前
吴媛媛完成签到 ,获得积分10
2秒前
FancyShi完成签到,获得积分10
2秒前
ZXneuro完成签到,获得积分10
2秒前
潘若宇大帅逼完成签到 ,获得积分10
3秒前
玖Nine完成签到,获得积分10
3秒前
3秒前
ZX612完成签到,获得积分10
4秒前
Zippon完成签到,获得积分10
4秒前
5秒前
6秒前
啵啵完成签到,获得积分10
6秒前
心灵的守望完成签到,获得积分10
7秒前
7秒前
gslscuer完成签到,获得积分10
7秒前
jia完成签到,获得积分10
7秒前
俭朴的世界完成签到 ,获得积分10
8秒前
传奇3应助hbydyy采纳,获得10
8秒前
8秒前
Arloong完成签到,获得积分10
9秒前
10秒前
完美的发卡完成签到,获得积分10
10秒前
平常亦凝发布了新的文献求助10
10秒前
seusyy完成签到,获得积分10
11秒前
tony完成签到,获得积分10
11秒前
聂学雨完成签到,获得积分10
11秒前
躲避问我发布了新的文献求助10
11秒前
12秒前
清爽的采萱完成签到,获得积分10
12秒前
12秒前
莫知完成签到,获得积分10
13秒前
Lily发布了新的文献求助10
13秒前
小飞机完成签到,获得积分10
14秒前
聂学雨发布了新的文献求助10
14秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3134083
求助须知:如何正确求助?哪些是违规求助? 2784882
关于积分的说明 7769151
捐赠科研通 2440425
什么是DOI,文献DOI怎么找? 1297383
科研通“疑难数据库(出版商)”最低求助积分说明 624959
版权声明 600792