Bone Marrow Stromal Cell-Derived Exosomes Promote Muscle Healing Following Contusion Through Macrophage Polarization

体内 炎症 间质细胞 纤维化 病理 骨骼肌 骨髓 脂多糖 巨噬细胞 生物 体外 医学 免疫学 解剖 生物化学 生物技术
作者
Zhiwen Luo,Jinrong Lin,Yaying Sun,Chenghui Wang,Jiwu Chen
出处
期刊:Stem Cells and Development [Mary Ann Liebert]
卷期号:30 (3): 135-148 被引量:50
标识
DOI:10.1089/scd.2020.0167
摘要

Skeletal muscle contusion is among the most common injuries in traumatology and clinics of sports medicine. The injured muscle is vulnerable to re-injury owing to fibrosis formation. Given that the bone marrow stromal cell-derived exosomes (BMSC-Exos) displayed promising therapeutic effect for various tissues, we used BMSC-Exos to treat skeletal muscle contusion and investigated its effects on muscle healing. In this study, the in vivo model of skeletal muscle contusion was established by subjecting the tibialis anterior of young male mice to hit injury, and the in vitro inflammation model was established by lipopolysaccharide treatment on macrophages. Macrophage depletion model was built by intraperitoneal injection with clodronate-containing liposomes. Exosomes were isolated and purified from the supernatant of BMSCs using gradient centrifugation. Nanoparticle tracking analysis, transmission electron microscope, and western blot were used to identify the exosomes. HE stain, Masson stain, immunofluorescence, and biomechanical testing were carried out on the muscle tissue. In addition, enzyme-linked immunosorbent assay (ELISA) assays, real-time qPCR, flow cytometry, and PKH67 fluorescence trace were conducted in vitro. Intramuscular injection of BMSC-Exos to mice after muscle contusion alleviated inflammation level, reduced fibrosis size, promoted muscle regeneration, and improved biomechanical property. After macrophages depletion, the effects of BMSC-Exos were inhibited. In vitro, PKH-67 fluorescence was internalized into macrophages. BMSC-Exos promoted M2 macrophages polarization both in vivo and in vitro. At the same time, BMSC-Exos reduced the production of inflammatory cytokines under the inflammatory microenvironment and upregulated anti-inflammatory factors expression. In conclusion, BMSC-Exos attenuated muscle contusion injury and promoted muscle healing in mice by modifying the polarization status of macrophages and suppressing the inflammatory reaction.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
basket完成签到 ,获得积分10
1秒前
贝壳发布了新的文献求助10
6秒前
陶醉的翠霜完成签到 ,获得积分10
6秒前
天行健完成签到,获得积分10
8秒前
苗槐完成签到,获得积分10
9秒前
罗氏集团完成签到,获得积分10
10秒前
良辰应助科研通管家采纳,获得10
18秒前
36456657应助科研通管家采纳,获得10
18秒前
天天快乐应助科研通管家采纳,获得10
18秒前
大地完成签到,获得积分10
21秒前
风信子完成签到,获得积分10
23秒前
rita_sun1969完成签到,获得积分10
24秒前
paopao完成签到 ,获得积分10
25秒前
看文献完成签到,获得积分10
25秒前
reset完成签到 ,获得积分10
27秒前
cata完成签到,获得积分10
28秒前
yuanletong完成签到 ,获得积分10
31秒前
dreamdraver完成签到,获得积分10
32秒前
fyjlfy完成签到 ,获得积分10
32秒前
NexusExplorer应助超帅的凌翠采纳,获得10
33秒前
贾舒涵完成签到,获得积分10
34秒前
jane完成签到 ,获得积分10
34秒前
大可完成签到 ,获得积分10
34秒前
在望完成签到 ,获得积分10
35秒前
幼荷完成签到 ,获得积分10
35秒前
Kai完成签到 ,获得积分10
35秒前
LiChard完成签到 ,获得积分10
39秒前
何兴棠完成签到,获得积分10
40秒前
贝壳完成签到,获得积分10
42秒前
hml123完成签到,获得积分10
45秒前
火山完成签到 ,获得积分10
46秒前
张尧摇摇摇完成签到 ,获得积分10
46秒前
小燚完成签到 ,获得积分10
47秒前
Anna完成签到,获得积分10
47秒前
byby完成签到,获得积分10
49秒前
马喽完成签到 ,获得积分10
50秒前
易哲完成签到 ,获得积分10
50秒前
也是难得取个名完成签到 ,获得积分10
50秒前
她的城完成签到,获得积分0
52秒前
皮半鬼完成签到,获得积分10
52秒前
高分求助中
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger Heßler, Claudia, Rud 1000
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 1000
Natural History of Mantodea 螳螂的自然史 1000
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
Barge Mooring (Oilfield Seamanship Series Volume 6) 600
ANSYS Workbench基础教程与实例详解 500
Spatial Political Economy: Uneven Development and the Production of Nature in Chile 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3326829
求助须知:如何正确求助?哪些是违规求助? 2957144
关于积分的说明 8583504
捐赠科研通 2635063
什么是DOI,文献DOI怎么找? 1442353
科研通“疑难数据库(出版商)”最低求助积分说明 668210
邀请新用户注册赠送积分活动 655103