[Establishing and identifying insulin resistance 3T3-L1 adipocytes cell model].

OBJECTIVE To establish insulin-resistant 3T3-L1 adipocytes cell model. METHODS 3T3-L1 preadipocytes were cultured and induced to differentation. We established insulin resistant 3T3-L1 induced by dexamethasone and determined the change of glucose concentration in cell culture. RESULTS In high glucose DMEM culture media, the glusose concentration significantly decreased. Achieved the peak of insulin-resisitant at the 96th hour induced by dexamethasone, and at this time compared with the blank the mRNA expression of Resistin of the model step up about three times. CONCLUSION 3T3-L1 adipocyte exposed to 1 micromol/L dexamethasone for 24 hours are able to induce a state of insulin resistance which can be maintained for 216 hours.