Development of new methods for the diagnosis of nasopharyngeal carcinoma

Objective Development of new methods, ELISA and immunostrip test, for the diagnosis of nasopharyngeal carcinoma. Methods The engineering purified antigens coat plate or absorb on nitrocellulose filter. The plate and diagnostic strips carrying antigens were used for detection of IgG antibody in the sera from nasopharyngeal carcinoma patients and outpatients patients. Results 127 cases sera from nasopharyngeal carcinoma patients were parallel detected TK/ IgG antibody by ELISA and immunostrips. The TK/IgG antibody are all positive in the 127 cases of nasopharyngeal carcinoma patients. 55 cases show positive by ELISA, 58 cases positive by immunostrips in 247 cases sera from outpatient. The antibody positive rate to early antigen p54 lower then to TK. Conclusion ELISA and imuunostrips are sensitive and specific means for detection of the IgG antibody to TK of EBV and the diagnosis of nasopharyngeal carcinoma. Key words: Herpesvirus 4, human; Thymine ; Serologic tests ; Nasapharyngeal neoplasms