[Significance of miR-155, miR-34a and miR-30a expression in diffuse large B-cell lymphoma].

Objective To determine the expressions of miR-155,miR-34a and miR-30a in diffuse large B-cell lymphoma and to explore their potential correlation with clinicopathological characteristics.Methods The expression level of miR 155,miR-34a and miR-30a in 46 DLBCL samples were determined with TaqMan real-time polymerase chain reaction.Interphase fluorescence in situ hybridization (I-FISH) was performed to detect MYC and p53 gene status and immunohistochemistry (Envision method) was used to evaluate the expression of CD3,CD10,CD20,BCL-6 and MUM-1 in DLBCL.The DLBCLs were classified into germinal center B cell-like (GCB) and non-germinal center B cell-like (non-GCB) subtypes according to Hans' criteria.Results Compared with normal controls,miR-155 expression level was significantly higher in DLBCL.The expression level of miR-155 in non-GCB type was higher than that in GCB type.It was shown that the patients with MYC rearrangement had lower expression level of miR-155 than the negative controls.Compared with p53 normal group,the expression level of miR-34a was significantly lower in p53 deletion group.It was also shown that the patients with BCL-6 protein positive had lower expression level of miR-30a than the negative group.Conclusion miR-155 expression level is different in normal controls,DLBCL and patients of subtype DLBCL.It therefore has a diagnosis value for DLBCL.miR-34a is of great prognostic significance.miR-155,miR-34a and miR-30a may be potential therapy targets for DLBCL. Key words: Diffuse large-cell lymphoma;  Immunophenotype;  MicroRNA;  Gene expression