脂肪酶
化学
皱纹假丝酵母
十二烷基硫酸钠
肺表面活性物质
疏水效应
对接(动物)
酶分析
色谱法
有机化学
核化学
酶
生物化学
医学
护理部
作者
Rui Zhang,Yang Liu,Xinran Huang,Mengchen Xu,Rutao Liu,Wansong Zong
标识
DOI:10.1016/j.scitotenv.2017.11.305
摘要
The extensive use of surfactants in food, laundry products and agriculture has caused concern about their biosafety. However, few studies have been done on their potential effect on the lipase which has always been used with surfactants in food and laundry industry. Herein, we investigated the interaction of three surfactants (sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS), sodium lauryl sulfonate (SLS)) with Candida rugosa lipase (CRL), which is a popular biocatalyst used regularly with surfactants. The effect of the three surfactants on the conformation and activity of CRL was evaluated by using multiple spectral methods, enzyme activity assay and molecular docking modeling. The results demonstrated that CRL interacted with SDS, SDBS and SLS primarily through hydrophobic forces, H-bonding and electrostatic forces, respectively. The binding constants (KA) of SDBS with CRL varied with temperature: 1.99 × 103 mol/L at 298 K and 4.13 × 103 mol/L at 318 K. SDS and SDBS affected the secondary structure and skeleton of CRL, which changed the polarity of CRL and enhanced its activity. SLS also changed the secondary structure and activity of CRL moderately, but had little effect on its polarity and chromophore microenvironment. Accordingly, all three surfactants exhibited effect to CRL on the molecular level calling for more attention to pay on their biosafety. The work demonstrates that SDS, SDBS and SLS could cause negative effects to CRL from different angles and therefore are not bio-friendly detergents.
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