拉伤
水解
降级(电信)
假单胞菌
化学
生物降解
细菌菌株
转化(遗传学)
双键
细菌
立体化学
生物化学
核化学
有机化学
生物
基因
解剖
电信
遗传学
计算机科学
作者
Gao-Jie Sun,Mingliang Zhang,Xiaoan Liu,Qinqin Gao,Wankui Jiang,Yidong Zhou,Hui Wang,Mengdi Cui,Jiguo Qiu,Jianhong Xu,Qing Hong
标识
DOI:10.1021/acs.jafc.8b06155
摘要
In this study, we isolated and characterized the bacterial strain Pseudomonas sp. BYT-1, which is capable of degrading pymetrozine and using it as the sole carbon source for growth. Strain BYT-1 could degrade 2.30 mM pymetrozine within 20 h under the optimal conditions of 30 °C and pH 7.0. Investigation of the degradation pathway showed that pymetrozine was oxidatively hydrolyzed to 4-amino-6-methyl-4,5-dihydro-2 H-[1,2,4]triazin-3-one (AMDT) and nicotinic acid (NA). The former accumulates as the end product in the culture, whereas the latter was hydroxylated to 6-hydroxynicotinic acid (6HNA) and subjected to further degradation. The transformation of pymetrozine to AMDT and NA by the cell-free extracts of strain BYT-1 also supported that the oxidative hydrolysis of the C═N double bond in pymetrozine was the initial degradation step. This is the first report on a pure bacterial culture with the ability to degrade pymetrozine. These findings enhance our understanding of the microbial degradation mechanism of pymetrozine.
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