胚芽
轴突
生物
前肢
再生(生物学)
命运图
祖细胞
细胞生物学
肢芽
重编程
胚胎干细胞
祖细胞
脊椎动物
干细胞
解剖
细胞
遗传学
基因
胚胎
作者
Tobias Gerber,Prayag Murawala,Dunja Knapp,Wouter Masselink,Maritta Schuez,Sarah Hermann,Malgorzata Gac-Santel,Sergej Nowoshilow,Jorge Kageyama,Shahryar Khattak,Joshua D. Currie,J. Gray Camp,Elly M. Tanaka,Barbara Treutlein
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2018-09-27
卷期号:362 (6413)
被引量:336
标识
DOI:10.1126/science.aaq0681
摘要
Amputation of the axolotl forelimb results in the formation of a blastema, a transient tissue where progenitor cells accumulate prior to limb regeneration. However, the molecular understanding of blastema formation had previously been hampered by the inability to identify and isolate blastema precursor cells in the adult tissue. We have used a combination of Cre-loxP reporter lineage tracking and single-cell messenger RNA sequencing (scRNA-seq) to molecularly track mature connective tissue (CT) cell heterogeneity and its transition to a limb blastema state. We have uncovered a multiphasic molecular program where CT cell types found in the uninjured adult limb revert to a relatively homogenous progenitor state that recapitulates an embryonic limb bud-like phenotype including multipotency within the CT lineage. Together, our data illuminate molecular and cellular reprogramming during complex organ regeneration in a vertebrate.
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