Epigallocatechin gallate ameliorates airway inflammation by regulating Treg/Th17 imbalance in an asthmatic mouse model

脾细胞 免疫学 医学 嗜酸性粒细胞 卵清蛋白 炎症 免疫球蛋白E 支气管肺泡灌洗 RAR相关孤儿受体γ 哮喘 FOXP3型 免疫系统 过敏性炎症 内科学 抗体
作者
Nan Yang,Yunxiao Shang
出处
期刊:International Immunopharmacology [Elsevier]
卷期号:72: 422-428 被引量:30
标识
DOI:10.1016/j.intimp.2019.04.044
摘要

Epigallocatechin gallate (EGCG) is a polyphenol that is found in green tea that has been shown to ameliorate airway inflammation in an ovalbumin-sensitized asthmatic mouse model. The purpose of this study was to investigate whether the immunomodulatory and anti-inflammatory effects of EGCG by regulating the regulatory T cell (Treg)/Th 17 cells balance in this model. Female BALB/c mice were sensitized and challenged with ovalbumin by intraperitoneal injection. EGCG was administered to asthmatic mice intraperitoneally 1 h before each OVA challenge. Airway hyperresponsiveness (AHR) was measured, and lung inflammatory infiltrations were assessed by hematoxylin and eosin (HE) staining. Serum OVA-specific IgE levels, Interleukin-10 (IL-10) levels and Interleukin-17A (IL-17A) levels in the bronchoalveolar lavage fluid (BALF), serum, and splenocyte culture supernatants were measured by ELISA. Flow cytometry was used to assess the effects of EGCG on the frequency of CD4+CD25+Foxp3+Treg cells in the splenocytes and real-time PCR method was used to measure the expression of Forkhead box P3 (Foxp3) mRNA and retinoid-related orphan receptor gammat (RORγt) mRNA in the lung tissue. The results showed that administration of EGCG significantly decreased AHR and OVA specific IgE in the serum, increased IL-10 levels in the BALF, serum, and splenocyte culture supernatant, and the frequency of CD4+CD25+Foxp3+Treg cells in the splenocytes in asthmatic mice. Administration of EGCG also ameliorated airway inflammation and eosinophil infiltrations in asthmatic mice. These results suggested that EGCG likely ameliorated OVA-induced airway inflammation by increasing the production of IL-10, the number of CD4+CD25+Foxp3+Treg cells and expression of Foxp3 mRNA in the lung tissue, and it could be an effective agent for treating asthma.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
Brendan完成签到,获得积分10
2秒前
即将高产sci完成签到,获得积分10
3秒前
简单刺猬发布了新的文献求助10
4秒前
蛋妞儿完成签到,获得积分10
4秒前
5秒前
卢西奥完成签到,获得积分10
6秒前
8秒前
DearWhite发布了新的文献求助30
9秒前
9秒前
10秒前
考拉发布了新的文献求助10
12秒前
Dr发布了新的文献求助10
14秒前
14秒前
端庄谷南完成签到 ,获得积分10
14秒前
junjun完成签到,获得积分10
15秒前
cugwzr完成签到,获得积分10
15秒前
湘君完成签到,获得积分0
17秒前
科研通AI2S应助心随以动采纳,获得10
17秒前
18秒前
红绿灯的黄完成签到,获得积分10
18秒前
19秒前
DearWhite完成签到,获得积分10
19秒前
haikuotian应助103921wjk采纳,获得20
20秒前
小西发布了新的文献求助10
21秒前
jjjjjjjjjjj发布了新的文献求助10
21秒前
21秒前
SciGPT应助zzz采纳,获得10
21秒前
LIKUN发布了新的文献求助10
22秒前
粥粥发布了新的文献求助10
22秒前
TTTHANKS完成签到 ,获得积分10
22秒前
可爱的函函应助粥粥采纳,获得10
28秒前
大个应助细心的抽屉采纳,获得10
30秒前
SciGPT应助自然的初丹采纳,获得10
31秒前
31秒前
Ava应助sfxnxgu采纳,获得10
31秒前
LMZ完成签到,获得积分10
31秒前
31秒前
31秒前
高分求助中
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Handbook of Qualitative Cross-Cultural Research Methods 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
Chen Hansheng: China’s Last Romantic Revolutionary 500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3139146
求助须知:如何正确求助?哪些是违规求助? 2790083
关于积分的说明 7793577
捐赠科研通 2446452
什么是DOI,文献DOI怎么找? 1301175
科研通“疑难数据库(出版商)”最低求助积分说明 626106
版权声明 601102