Tungsten Oxide Nanospheres Garnished on Functionalized Chitosan Biocomposite: A Sensitive Detection for Melatonin

Primitive detection of melatonin (Mel) is in great demand for human body regulation and cancer diagnosis. Recently natural polymer based biocomposites are gaining attraction owing to their specific functionalities attracting the innovative module. In this sense, a selective and sensitive biosensing of Mel using Tungsten oxide (WO3) nanospheres decorated with functionalized chitosan (FCH) is investigated in this paper. Functionalization of chitosan produces excess of amine groups generating interhydrogen bonding between the FCH and WO3, thus provides necessary stability for WO3/FCH biocomposite formation which is confirmed by primary physical characterizations. Also, electrochemical analysis suggests the excellent electrocatalytic performance of the prepared biocomposite towards Melatonin (Mel) shows linear range of 50 nM - 1 mM with limit of detection 4.9 nM with R 2 = 0.996. Further the selectivity, reproducibility and stability propounds the exceptional biosensing assets of the proposed biocomposite. Finally the practical reliability of the preferred biocomposite was evaluated in serum and urine sample for real sample analysis with satisfactory results. Furthermore, the exceptional properties along with admiring results put forward this biosensor for device fabrication and point of care devices in futuristic clinical and industrial Mel sensing applications.