Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E

Phylogenetic analyses of ARF family GTPases predict that ARL16 is linked to cilia. This was confirmed using mouse embryonic fibroblasts deleted for ARL16, resulting in defects in Golgi-to-cilium traffic, with accumulation of IFT140 and INPP5E at Golgi.