摘要
CytometryVolume 14, Issue 5 p. 486-491 Special ReportFree Access Consensus review of the clinical utility of dna flow cytometry in colorectal cancer† Kenneth D. Bauer, Corresponding Author Kenneth D. Bauer Department of Cell Analysis, Genentech, Inc., South San Francisco, California 94080Department of Cell Analysis, Genentech, Inc., 460 Pt. San Bruno Boulevard, South San Francisco, CA 94080Search for more papers by this authorC. Bruce Bagwell, C. Bruce Bagwell Verity Software House, Topsham, Maine 04086Search for more papers by this authorWalter Giaretti, Walter Giaretti National Cancer Institute, Biophysics Laboratory, Genoa, ItalySearch for more papers by this authorMyron Melamed, Myron Melamed Department of Pathology, New York Medical College, Valhalla, New York 10595Search for more papers by this authorRichard J. Zarbo, Richard J. Zarbo Department of Pathology, Henry Ford Hospital, Detroit, Michigan 48202Search for more papers by this authorThomas E. Witzig, Thomas E. Witzig Department of Hematology and Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905Search for more papers by this authorPeter S. Rabinovitch, Peter S. Rabinovitch Department of Pathology, University of Washington, Seattle, Washington 98195Search for more papers by this author Kenneth D. Bauer, Corresponding Author Kenneth D. Bauer Department of Cell Analysis, Genentech, Inc., South San Francisco, California 94080Department of Cell Analysis, Genentech, Inc., 460 Pt. San Bruno Boulevard, South San Francisco, CA 94080Search for more papers by this authorC. Bruce Bagwell, C. Bruce Bagwell Verity Software House, Topsham, Maine 04086Search for more papers by this authorWalter Giaretti, Walter Giaretti National Cancer Institute, Biophysics Laboratory, Genoa, ItalySearch for more papers by this authorMyron Melamed, Myron Melamed Department of Pathology, New York Medical College, Valhalla, New York 10595Search for more papers by this authorRichard J. Zarbo, Richard J. Zarbo Department of Pathology, Henry Ford Hospital, Detroit, Michigan 48202Search for more papers by this authorThomas E. Witzig, Thomas E. Witzig Department of Hematology and Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905Search for more papers by this authorPeter S. Rabinovitch, Peter S. Rabinovitch Department of Pathology, University of Washington, Seattle, Washington 98195Search for more papers by this author First published: 1993 https://doi.org/10.1002/cyto.990140506Citations: 83 † These consensual guidelines and recommendations address the potential utility of DNA cytometry in characterizing human malignancies. They are provided to inform laboratory personnel, pathologists, and clinicians about DNA cytometry. For individual patients, use of DNA cytometry, selection of specific techniques, and interpretation and utilization of results remains the responsibility of the attending physicians. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Literature Cited 1 Boring CC, Squires TS, Tong T: Cancer Statistics, 1992. CA 42: 19–38, 1992. 2 Wolley RC, Schreiber K, Koss LG, Karas M, Sherman A: DNA distribution in human colon carcinomas and its relationship to clinical behavior. J Natl Cancer Inst 69: 15–22, 1982. 3 Melamed M, Enker W, Banner P, Janov A, Kessler G, Darzynkiewicz Z: Flow cytometry of colorectal carcinoma with three-year follow-up. Dis Colon Rectum 29: 184–186, 1986. 4 Emdin A, Stenling R, Roos G: Prognostic value of DNA content in colorectal carcinoma. Cancer 60: 1282–1287, 1987. 5 Wirsching RP, Lamerz R, Wiebecke B, Demmel N, Liewald F, Valet G: Flow cytometric evaluation of colorectal carcinoma as completion of conventional tumor examination. J Exp Clin Cancer Res 6: 117–128, 1987. 6 Rognum T, Lund E, Meling G, Langmark F: Near diploid large bowel carcinomas have better five-year survival than aneuploid ones. Cancer 68: 1077–1081, 1991. 7 Enker WE, Kimmel M, Cibas ES, Cranor ML, Melamed MR: DNA/RNA content and proliferative fractions of colorectal carcinomas: A five-year prospective study relating flow cytometry to survival. J Natl Cancer Inst 83: 701–708, 1991. 8 Giaretti W, Danova M, Deido E, Mazzini G, Sciallero S, Aste H, Scivetti P, Riccardi A, Marsano B, Mero F, d-Amore E: Flow cytometric DNA index in the prognosis of colorectal cancer. Cancer 67: 1921–1927, 1991. 9 Armitage NC, Robins RA, Evans DV, et al: The influence of tumor DNA abnormalities in colorectal cancer. Br J Surg 72: 828–830, 1985 10 Kokal W, Sheibani K, Terz J, Harada R: Tumor DNA content in the prognosis of colorectal carcinoma. JAMA 255: 3123–3127, 1986. 11 Scott NA, Rainwater LM, Wieand HS, Weiland LH, Pemberton JH, Beart RW, Lieber MM: The relative prognostic value of flow cytometric DNA analysis and conventional clinicopathologic criteria in patients with operable rectal carcinomas. Dis Colon Rectum 30: 513–520, 1987. 12 Scott NA, Wieand HS, Moertel CG, Cha SS, Beart RW, Lieber MM: Colorectal cancer. Dukes stage, tumor site, preoperative plasma CEA level, and patient prognosis related to tumor DNA ploidy pattern. Arch Surg 122: 1375–1379, 1987. 13 Quirke P, Dixon M, Clayden A, Durdey P, Dyson J, Williams N, Bird C: Prognostic significance of DNA aneuploidy and cell proliferation in rectal adenocarcinoma. J Pathol 151: 285–291, 1987. 14 Bauer KD, Lincoln S, Vera-Roman J, Chmiel J, Madurski M, Murad T, Scarpelli D: Prognostic implications of proliferative activity and DNA aneuploidy in colonic adenocarcinomas. Lab Invest 57: 329–335, 1987. 15 Schutte B, Reynders M, Wiggers T, Arends J, Volovics L, Bosman F, Blijham G: Retrospective analysis of the prognostic significance of DNA content and proliferative activity in large bowel carcinoma. Cancer Res 47: 329–335, 1987. 16 Goh HS, Jass JR, Atkin WS, Cuzick J, Northover JMA: Value of flow cytometric determination of ploidy as a guide to prognosis in rectal cancer: A multivariate analysis. Int J Colorectal Dis 2: 17–21, 1987. 17 Jones DJ, Moore M, Schofield PF: Prognostic significance of DNA ploidy in colorectal cancer: A prospective flow cytometric study. Br J Surg 75: 28–33, 1988. 18 Wiggers T, Arends J, Schutte B, Volovics L, Bosman F: A multivariate analysis of pathologic prognostic indicators in large bowel cancer. Cancer 61: 386–395, 1988. 19 Fisher E, Siderits R, Sass R, Fisher B: Value of assessment of ploidy in rectal cancers. Arch Path Lab Med 113: 525–528, 1989. 20 Halvorsen T, Johannesen E. DNA ploidy tumour site, and prognosis in colorectal cancer: A flow cytometric study of paraffin-embedded tissue. Scand J Gastroenterol 25: 141–148, 1990. 21 Visscher DW, Zarbo RJ, Ma CK, Sakr WA, Crissman JD: Flow cytometric DNA and clinicopathologic analysis of Dukes' A and B colonic adenocarcinomas: A retrospective study. Modern Pathol 3: 709–712, 1990. 22 Harlow S, Eriksen B, Poggensee L, Chmiel J, Scarpelli D, Murad T, Bauer K: Prognostic implications of proliferative activity and DNA aneuploidy in Astler-Coller Dukes stage C colonic adenocarcinomas. Cancer Res 51: 2403–2409, 1991. 23 Witzig T, Loprinzi C, Gonchoroff N, Reiman H, Cha S, Weiand H, Katzman J, Paulsen J, Moertel C: DNA ploidy and cell kinetic measurements as predictors of recurrence and survival in stages B2 and C colorectal adenocarcinomas. Cancer 68: 879–888, 1991. 24 Jass JR, Mukawa K, Love SB, Capellaro D: Clinical importance of DNA content in rectal cancer measured by flow cytometry. J Clin Pathol 42: 254–259, 1989. 25 Armitage N, Ballantyne K, Sheffield J, Clarke P, Evans D, Hardcastle J: A prospective evaluation of the effect of tumor cell DNA content on recurrence in colorectal cancer. Cancer 67: 2599–2604, 1991. 26 Zarbo RJ, Linden MD: DNA analysis of gastrointestinal and genitourinary tract neoplasms. In: Flow cytometry in clinical diagnosis, 2nd ed, DF Keren, H Hanson, P Hurtubise (eds). American Society of Clinical Pathologists, Chicago, (in press). 27 Hiddeman W., von Bassewitz DB, Kleinemeier HJ, Schulte-Brochterbeck E, Hauss J, Lingemann B, Buchner T, Grundman E: DNA stemline heterogeneity in colorectal cancer. Cancer Res 58: 258–263, 1986. 28 Giaretti W and Santi L: Tumor progression by DNA flow cytometry in human colorectal cancer. Intl J Cancer 45: 597–603, 1990. 29 Petersen SE, Bichel P, Lorentzen M: Flow cytometric demonstration of tumor-cell subpopulations with different DNA contents in human colorectal carcinomas. Eur J Cancer 15: 383–386, 1978. 30 Wersto RP, Liblit RL, Deitch D, Koss LG: Variability in DNA measurements in multiple samples of human colonic carcinoma. Cancer 67: 106–115, 1991. 31 Petersen SE, Lorentzen M, Bichel P: A mosaic subpopulation structure of human colorectal carcinomas demonstrated by flow cytometry. In: Flow Cytometry, 4th ed, OD Laerum, T Lindmo, E Thorud (eds). Universitetsforlaget, Bergen, 1980, pp 412–416. 32 Rognum TO, Thorud E, Elgjo K, Brandtzaeg P, Orjasaeter H, Nygaard K: Large-bowel carcinomas with different ploidy, related to secretory component, IgA- and CEA and plasma. Br J Cancer 45: 921–934, 1982. 33 Tribukait B, Hammarberg C, Rubio C: Ploidy and proliferation patterns in colorectal adenocarcinomas related to Dukes' classification and to histopathological differentiation. Acta Pathol Microbiol Scand (A) 91: 89–95, 1983. 34 Frankfurt OS, Slocum HK, Rustum YM, et al: Flow cytometric analysis of DNA aneuploidy in primary and metastatic human solid tumors. Cytometry 5: 71–80, 1984. 35 Scott NA, Grande JP, Weiland LH, et al: Flow cytometric DNA patterns from colorectal cancers—how reproducible are they? Mayo Clin Proc 62: 331–337, 1987. 36 Crissman J, Zarbo R, Ma C, Visscher D: Histopathologic parameters and DNA analysis in colorectal adenocarcinomas Pathol. Annual 24 (Part 2): 103–147, 1989. 37 van den Ingh HF, Griffioen G, Cornelisse CJ: DNA aneuploidy in colorectal adenomas. Br. J. Cancer 55: 351–354, 1987. 38 Dean PN: In: Flow Cytometry: Instrumentation and Data Analysis. MA Van Dilla, PN Dean, OD Laerum, MR Melamed (eds). Academic Press, San Diego, 1985, pp 195–221. 39 Darzynkiewicz Z, Traganos F, Kapuscinski J, Staiano-Coico L, Melamed MR: Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry 5: 355–363, 1984. 40 Bauer KD: Quality control issues in DNA content flow cytometry. In: Clinical Flow Cytometry, Landay AL, Ault KA, Bauer KD, Rabinovitch PS (eds). Annals NY Acad. Sci. 677: 59–77, 1993. 41 Wolley RC, Herz F, Koss LG: Caution on the use of lymphocyte standards in the flow cytometric analysis of cultured cells. Cytometry 2: 370–373, 1982. 42 Rabinovitch P. S. Practical considerations for DNA content and cell cycle analysis. In: Clinical Flow Cytometry: Principles and Application, KD Bauer, RE Duque, TV Shankey (eds). Williams and Wilkins, Baltimore, 1993, pp 117–142. 43 Moertel CG, Loprinzi CL, Witzig TE, Wieand HS, Gonchoroff NJ, Reiman HM, Galandiuk S, Fitzgibbons RJ, Barlow JF, Donchue JH: Proceedings, 26th Annual Meeting, American Society for Clinical Oncology, 1990. 44 Benson III, AB, Bauer KD, Lefkopoulow M, Murad T, Rao S, Wolf B, Hammes L, Haller D: Proceedings, 83rd Annual Meeting, American Association for Cancer Research, 1992. 45 Lofberg R, Brostrom O, Karlen P, Tribukait B, Ost A: Colonoscopic surveillance in long standing ulcerative colitis—1:15-year follow-up study. Gastroenterology 99: 1021–1031, 1990. 46 Levine DS, Rabinovitch PS, Haggitt RC, Blount PL, Dean PJ, Rubin CE, Reid BJ: Distribution of aneuploid cell populations in ulcerative colitis with dysplasia or cancer. Gastroenterology 101: 1198–1210, 1991. 47 Fischbach W, Mossner J, Seyschab H, Hohn H: Tissue carcinoembryonic antigen and DNA aneuploidy in precancerous and cancerous colorectal lesions. Cancer 65: 1820–1824, 1990. 48 Melville DM, Jass JR, Shepard NA, Northover JMA, Capallero D, Richman PI, Lennard-Jones JE, Richie JK, Andersen SN: Dysplasia and deoxyribonucleic acid aneuploidy in the assessment of precancerous changes in chronic ulcerative colitis: Observer variations and correlations. Gastroenterology 95: 668–675, 1989. 49 Rubin CE, Haggitt RC, Burmer GC, et al.: DNA aneuploidy in colonic biopsies predicts future development of dysplasia in ulcerative colitis. Gastroenterology, 103: 1611–1620, 1992. 50 Zarbo RJ, Visscher DW, Crissman JD: Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte common antigen. Anal Quant Cytol Histol 11: 391–402, 1989. 51 Bauer KD, Clevenger CV, Endow RK, Murad T, Epstein AL, Scarpelli DG: Simultaneous nuclear antigen and DNA content quantitation using paraffin-embedded colonic tissue and multiparameter flow cytometry. Cancer Res 46: 2428–2434, 1986. 52 Watson JV, Steward J, Evan GI, et al: The clinical significance of flow cytometric c- myc oncoprotein quantitation in testicular cancer. Br J Cancer 53: 331–337, 1986. 53 Krishan A, Sauerteig A: Flow cytometric monitoring of cellular resistance to cancer chemotherapy. In, RE Duque, TV Shankey (eds), Williams and Wilkins, Baltimore, pp 459–468, 1993. Citing Literature Volume14, Issue51993Pages 486-491 ReferencesRelatedInformation