Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B

血小板 整合素 细胞生物学 热休克蛋白 化学 血小板活化 污渍 细胞质 热休克蛋白70 生物化学 致密颗粒 分子生物学 生物 受体 免疫学 基因
作者
Chao Ma,Ying Yao,Qing-Xi Yue,Xuan‐Wei Zhou,Pengyuan Yang,Wanying Wu,Shu‐Hong Guan,Baohong Jiang,Min Yang,Xuan Li,De‐An Guo
出处
期刊:PLOS ONE [Public Library of Science]
卷期号:6 (2): e14692-e14692 被引量:35
标识
DOI:10.1371/journal.pone.0014692
摘要

Salvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin α2β1. But, the signal cascades in platelets after SB binding are still not clear.In the present study, a differential proteomic analysis (two-dimensional electrophoresis) was conducted to check the protein expression profiles of rat platelets with or without treatment of SB. Proteins altered in level after SB exposure were identified by MALDI-TOF MS/MS. Treatment of SB caused regulation of 20 proteins such as heat shock-related 70 kDa protein 2 (hsp70), LIM domain protein CLP-36, copine I, peroxiredoxin-2, coronin-1 B and cytoplasmic dynein intermediate chain 2C. The regulation of SB on protein levels was confirmed by Western blotting. The signal cascades network induced by SB after its binding with integrin α2β1 was predicted. To certify the predicted network, binding affinity of SB to integrin α2β1 was checked in vitro and ex vivo in platelets. Furthermore, the effects of SB on protein levels of hsp70, coronin-1B and intracellular levels of Ca²+ and reactive oxygen species (ROS) were checked with or without pre-treatment of platelets using antibody against integrin α2β1. Electron microscopy study confirmed that SB affected cytoskeleton structure of platelets.Integrin α2β1 might be one of the direct target proteins of SB in platelets. The signal cascades network of SB after binding with integrin α2β1 might include regulation of intracellular Ca²+ level, cytoskeleton-related proteins such as coronin-1B and cytoskeleton structure of platelets.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
smz完成签到 ,获得积分10
1秒前
2秒前
叮当完成签到,获得积分10
2秒前
bopbopbaby发布了新的文献求助10
3秒前
4秒前
FANGQUAN发布了新的文献求助10
4秒前
5秒前
6秒前
7秒前
7秒前
Crystal完成签到,获得积分10
8秒前
gyy完成签到,获得积分10
8秒前
霸气紫文应助ThoseRangers0624采纳,获得30
8秒前
端庄的梦山完成签到 ,获得积分10
9秒前
爱科研完成签到 ,获得积分10
10秒前
luoshikun发布了新的文献求助10
12秒前
思源应助nbing采纳,获得10
13秒前
13秒前
Wang完成签到,获得积分10
13秒前
14秒前
sss发布了新的文献求助10
14秒前
昏睡的天曼完成签到,获得积分20
15秒前
DIDIDI完成签到 ,获得积分10
15秒前
齐平露发布了新的文献求助10
15秒前
思源应助机器猫采纳,获得10
16秒前
无奈世立发布了新的文献求助10
16秒前
16秒前
16秒前
17秒前
忧郁平文完成签到,获得积分10
17秒前
丢丢发布了新的文献求助10
18秒前
海边听海完成签到 ,获得积分10
19秒前
bkagyin应助威武的迎彤采纳,获得10
19秒前
华仔应助小陈采纳,获得10
19秒前
1007完成签到,获得积分10
19秒前
orixero应助TristeOwen采纳,获得10
19秒前
20秒前
qqym完成签到,获得积分10
22秒前
天真的嚓茶完成签到,获得积分10
22秒前
坤坤发布了新的文献求助10
22秒前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Cognitive Paradigms in Knowledge Organisation 2000
Effect of reactor temperature on FCC yield 2000
Introduction to Spectroscopic Ellipsometry of Thin Film Materials Instrumentation, Data Analysis, and Applications 1200
How Maoism Was Made: Reconstructing China, 1949-1965 800
Barge Mooring (Oilfield Seamanship Series Volume 6) 600
Medical technology industry in China 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3312450
求助须知:如何正确求助?哪些是违规求助? 2945105
关于积分的说明 8522863
捐赠科研通 2620823
什么是DOI,文献DOI怎么找? 1433131
科研通“疑难数据库(出版商)”最低求助积分说明 664863
邀请新用户注册赠送积分活动 650231