玉米黄质
再结晶(地质)
化学
丙酮
色谱法
分馏
超临界流体
枸杞
溶剂
超临界二氧化碳
有机化学
叶黄素
生物化学
类胡萝卜素
生物
病理
古生物学
医学
替代医学
作者
Li-Pin Chang,Jiahui Cheng,Shih-Lan Hsu,Bing-Chung Liau,Tzong‐Ming Wu,Chieh-Ming J. Chang
标识
DOI:10.1016/j.supflu.2011.02.008
摘要
► Supercritical carbon dioxide anti-solvent recrystallization was employed in rapidly producing micro-sized zeaxanthin dipalmitates (ZP) as eye-sight protecting health supplements from Lycium barbarum fruits. ► De-glycosides pre-treatment was necessary in obtaining the purest ZP compound. ► Anti-oxidation ability of ARPE-19 cells was successfully examined for these ZP compounds. This study employed column elution fractionation coupled with supercritical anti-solvent recrystallization to isolate the purest zeaxanthin dipalmitates (ZP), one of the eye-protecting compounds in the Lycium barbarum fruits. The amount of ZP increased to 983 mg/g in liquid anti-solvent precipitates with 29.1% recovery and increased to 929 mg/g in supercritical anti-solvent precipitates with 59.6% recovery, respectively from the ultrasonic acetone extract. Experimental data showed that supercritical anti-solvent recrystallization is feasible in producing platelet type precipitates within a few minutes. These ZP compounds exhibit anti-oxidative abilities by proliferating human adult retinal pigment epithelial cells (from 22% to 31%); though it was a meager increase when compared to those of algal 95% zeaxanthin (from 43% to 94%).
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