脂多糖
免疫系统
炎症
生物
白细胞介素2受体
免疫学
体外
调节性T细胞
T细胞
生物化学
作者
R. Shanmugasundaram,Ramesh K. Selvaraj
标识
DOI:10.1016/j.vetimm.2011.08.016
摘要
The objective of this study was to identify the effect of in vitro lipopolysaccharide (LPS) treatment on regulatory T cells (Tregs) from chickens. Tregs had approximately 30-fold higher TLR 2-type 2 and six-fold higher TLR 4 mRNA content than CD4+CD25− cells. Tregs were treated with either 0 or 1 μg/ml LPS for 0, 2, and 4 d. LPS treatment increased the IL-2 mRNA amount in Tregs at 2 and 4 d post-LPS treatment. LPS treatment increased the IL-10 mRNA amount in Tregs at 4 d post-LPS treatment. The total live cell numbers were approximately two-fold higher at 2 d and three-fold higher at 4 d in the 1 μg/ml LPS-treated groups than in the 0 μg LPS-treated controls. LPS treatment abrogated suppressive properties of Tregs at 2 d post-LPS treatment. At 4 d post-LPS treatment, Tregs became supersuppressive. In conclusion, chicken Tregs are differentially activated to facilitate immune response during the early stage of inflammation and to facilitate immune suppression at a later stage of inflammation.
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