E. coli Expression and Purification of Fab Antibody Fragments

Abstract The Fab molecule was the first generated antibody fragment and still dominates basic research and clinical applications. This unit describes the E. coli expression and purification of Fab antibody fragments with and without a His tag, and is designed to yield sufficient protein for the evaluation and characterization of a panel of Fab selected from a Fab library by phage display. Curr. Protoc. Protein Sci . 55:6.10.1‐6.10.14. © 2009 by John Wiley & Sons, Inc.