Isolation of components with antimicrobial property from the donkey milk: a preliminary study.

驴子 抗菌剂 分离(微生物学) 生物 微生物学 食品科学 抗生素 青霉素
作者
Filomena Nazzaro,Pierangelo Orlando,Florinda Fratianni,Raffaele Coppola
出处
期刊:The Open Food Science Journal [Bentham Science]
卷期号:4 (1): 43-47 被引量:26
标识
DOI:10.2174/1874256401004010043
摘要

The presence of components in donkey milk, different from lysozyme, capable of inhibiting the growth of pathogenic microorganisms was investigated. Milk was acidified, hydrolyzed with pepsin, and analyzed by HPLC. The chromatographic fractions were collected in 5 main pools that, after the removal of lysozyme, were analyzed by micro- electrophoresis; antimicrobial activity was evaluated against the pathogens Bacillus cereus, Staphylococcus aureus, En- terococcus faecalis, and Escherichia coli. Hydrolyzed milk showed a protein pattern ranging from 0.78 to 25.2 kDa. It was active against all pathogenic strains tested, with inhibition halos ranging from 4.3 mm (against E.coli) to 17.4 mm (versus E.faecalis). Among the pools collected after HPLC step, pool b resulted to be the most rich in protein bands, most of them with a molecular weght less than 20.4 kDa, and the most effective against all the pathogens, except against E. coli. Pool a, was active versus E. coli and B. cereus and contained only two bands, at 0.31 and 8.3 kDa, this last band probably responsible for its antimicrobial activity and chemically different from the other ones with a similar MW present in pools c and e that did not inhibit the growth of the pathogenic strains. This finding highlights the presence of bio molecules released during proteolytic hydrolysis that may contribute to the antimicrobial activity in donkey milk and play a significant role in the host defense system.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
xcx发布了新的文献求助10
刚刚
天地一体完成签到,获得积分10
1秒前
坚定盈完成签到,获得积分10
2秒前
稀里糊涂完成签到 ,获得积分10
2秒前
多走一步完成签到,获得积分10
3秒前
w_完成签到,获得积分10
4秒前
爱听歌依波完成签到 ,获得积分10
4秒前
Leon发布了新的文献求助10
4秒前
gsgg完成签到 ,获得积分20
5秒前
笨笨友桃完成签到,获得积分10
5秒前
在水一方应助Huang采纳,获得10
5秒前
6秒前
7秒前
郭郭要努力ya完成签到 ,获得积分10
8秒前
9秒前
科研通AI2S应助Saint采纳,获得10
9秒前
annafan完成签到,获得积分10
9秒前
10秒前
科目三应助雷桑采纳,获得10
10秒前
少吃顿饭并不难完成签到 ,获得积分10
11秒前
珠珠发布了新的文献求助10
11秒前
11秒前
刘三哥完成签到 ,获得积分10
12秒前
隐形曼青应助leederay采纳,获得10
13秒前
上官若男应助to_ooooo采纳,获得10
13秒前
海阔天空发布了新的文献求助10
14秒前
顺心羊完成签到,获得积分10
14秒前
科研_小白完成签到,获得积分10
14秒前
XJTU_jyh完成签到,获得积分10
15秒前
TheaGao完成签到 ,获得积分10
15秒前
不是省油的灯完成签到 ,获得积分10
17秒前
19秒前
19秒前
bububu完成签到,获得积分10
20秒前
nanaki完成签到,获得积分10
20秒前
21秒前
小蘑菇应助lin采纳,获得10
22秒前
22秒前
23秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038619
求助须知:如何正确求助?哪些是违规求助? 3576294
关于积分的说明 11375058
捐赠科研通 3306084
什么是DOI,文献DOI怎么找? 1819374
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815066