Aggregation-induced electrochemiluminescence enhancement of Ag-MOG for amyloid β 42 sensing

This study aimed to introduce an immunosensor for measuring amyloid β 42 (Aβ42) levels by aggregation-induced enhanced electrochemiluminescence (ECL). Metal–organic gels (MOGs) are novel soft materials with advantages such as high gel stability, good light-emitting properties, and easy preparation. This study used silver nanoparticle metal–organic gel (Ag-MOG) as a substrate to connect Aβ42-Ab2 and the cathodoluminescent probe. Potassium persulfate was used as a co-reactant that could emit a high ECL signal. CuS@Au had the benefits of a relatively large surface area with excellent carrier function; therefore, it was used as a substrate to load a large amount of Aβ42-Ab1, significantly improving the immunosensor sensitivity. The ECL intensity of Aβ42 was linear in the range of 0.01 pg/mL to 250 ng/mL with a detection limit of 2.2 fg/mL (S/N = 3) under optimized detection conditions. This ECL immunosensor has been successfully applied to detect Aβ42 in human serum with the advantages of excellent stability and high selectivity. This method not only expands the potential applications of ECL immunosensors based on biological testing and clinical diagnosis but also provides a viable approach to basic clinical testing.