生物
花青素
MYB公司
向日葵
转录组
异位表达
向日葵
植物
类黄酮
基因
基因表达
拟南芥
向日葵
细胞生物学
遗传学
生物化学
园艺
突变体
抗氧化剂
作者
Wenhui Jiang,Qinqin Jiang,Zhijie Shui,Peipei An,Shandang Shi,Tianxiang Liu,Hanbing Zhang,Shuyi Huang,Bing Jing,Enshi Xiao,Li Quan,Jixia Liu,Zhonghua Wang
出处
期刊:Plant Science
[Elsevier]
日期:2024-01-01
卷期号:338: 111901-111901
被引量:2
标识
DOI:10.1016/j.plantsci.2023.111901
摘要
Sunflowers are well-known ornamental plants, while sunflowers with red corolla are rare and the mechanisms underlying red coloration remain unclear. Here, a comprehensive analysis of metabolomics and transcriptomics on flavonoid pathway was performed to investigate the molecular mechanisms underlying the differential color formation between red sunflower Pc103 and two yellow sunflowers (Yr17 and Y35). Targeted metabolomic analysis revealed higher anthocyanin levels but lower flavonol content in Pc103 compared to the yellow cultivars. RNA-sequencing and phylogenetic analysis identified multiple genes involved in the flavonoid pathway, including series of structural genes and three MYB and bHLH genes. Specifically, HaMYBA and HabHLH1 were up-regulated in Pc103, whereas HaMYBF exhibited reduced expression. HaMYBA was found to interact with HabHLH1 in vivo and in vitro, while HaMYBF does not. Transient expression analysis further revealed that HabHLH1 and HaMYBA cooperatively regulate increased expression of dihydroflavonol 4-reductase (DFR), leading to anthocyanin accumulation. On the other hand, ectopic expression of HaMYBF independently modulates flavonol synthase (FLS) expression, but hindered anthocyanin production. Collectively, our findings suggest that the up-regulation of HaMYBA and HabHLH1, as well as the down-regulation of HaMYBF, contribute to the red coloration in Pc103. It offers a theoretical basis for improving sunflower color through genetic engineering.
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