检出限
核酸外切酶 III
硫堇
线性范围
胶体金
适体
聚醚酰亚胺
纳米复合材料
石墨烯
材料科学
电化学
化学
纳米技术
组合化学
纳米颗粒
化学工程
色谱法
聚合物
电极
生物化学
复合材料
物理化学
工程类
大肠杆菌
遗传学
基因
生物
作者
Junjie Zhu,Baoshan He,Lingling Xie,Xiaoyu Cao,Ying Liang,Jinshui Wang
标识
DOI:10.1016/j.snb.2023.133542
摘要
A “signal off” electrochemical aptasensor based on metal-organic framework (MOF) - based nanocomposites and RecJf exonuclease-assisted target recycling was constructed for ultrasensitive detection of vanillin (VAN). In this work, benefited from the high catalytic activity of the synthesized gold nanoparticle/iron-based MOF/polyetherimide-reduced graphene oxide (AuNPs/NH2-MOF-235(Fe)/PEI-rGO) and the AuNPs/Ce-MOF nanocomposites toward thionine (Thi), the electrochemical signal was amplified. And the target recycling assisted by RecJf exonuclease were triggered with the presence of VAN, which induced the strongly reduced electrochemical signal. On account of dual amplification strategies, the performance of the aptasensor was significantly improved to achieve ultrasensitive detection of VAN. Under optimal conditions, the linear relationship was fitted for VAN with a linear range of 2 pM - 0.2 mM and the detection limit of 1.14 pM (S/N = 3). Furthermore, this assay for VAN has excellent specificity, long-term stability, and satisfactory results of the detection in the real samples. In summary, the aptasensor could be further used for the specific quantitative determination of other targets in foods.
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