二甲基磺酰丙酸盐
生物
ATP结合盒运输机
细菌
运输机
海洋噬菌体
生态学
计算生物学
遗传学
基因
浮游植物
营养物
作者
Chunyang Li,Michaela A. Mausz,Andrew R. J. Murphy,Nan Zhang,Xiu‐Lan Chen,Li Wang,Chao Gao,Maria del Mar Aguiló‐Ferretjans,Eleonora Silvano,Ian Lidbury,Huihui Fu,Jonathan D. Todd,Yin Chen,Yu‐Zhong Zhang
出处
期刊:The ISME Journal
[Springer Nature]
日期:2023-01-27
卷期号:17 (4): 579-587
被引量:14
标识
DOI:10.1038/s41396-023-01375-3
摘要
Abstract Dimethylsulfoniopropionate (DMSP) is a ubiquitous organosulfur compound in marine environments with important functions in both microorganisms and global biogeochemical carbon and sulfur cycling. The SAR11 clade and marine Roseobacter group (MRG) represent two major groups of heterotrophic bacteria in Earth’s surface oceans, which can accumulate DMSP to high millimolar intracellular concentrations. However, few studies have investigated how SAR11 and MRG bacteria import DMSP. Here, through comparative genomics analyses, genetic manipulations, and biochemical analyses, we identified an ABC (ATP-binding cassette)-type DMSP-specific transporter, DmpXWV, in Ruegeria pomeroyi DSS-3, a model strain of the MRG. Mutagenesis suggested that DmpXWV is a key transporter responsible for DMSP uptake in strain DSS-3. DmpX, the substrate binding protein of DmpXWV, had high specificity and binding affinity towards DMSP. Furthermore, the DmpX DMSP-binding mechanism was elucidated from structural analysis. DmpX proteins are prevalent in the numerous cosmopolitan marine bacteria outside the SAR11 clade and the MRG, and dmpX transcription was consistently high across Earth’s entire global ocean. Therefore, DmpXWV likely enables pelagic marine bacteria to efficiently import DMSP from seawater. This study offers a new understanding of DMSP transport into marine bacteria and provides novel insights into the environmental adaption of marine bacteria.
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