Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

壳聚糖 生物相容性 伤口愈合 体内 炎症 细胞毒性 促炎细胞因子 活力测定 体外 药理学 核化学 化学 材料科学 医学 免疫学 有机化学 生物技术 生物 生物化学
作者
Zhikang Zhu,Fang He,Huawei Shao,Jiaming Shao,Qiong Li,Xingang Wang,Haitao Ren,Chuangang You,Zhongtao Zhang,Chunmao Han
出处
期刊:ACS applied nano materials [American Chemical Society]
卷期号:6 (1): 573-587 被引量:14
标识
DOI:10.1021/acsanm.2c04714
摘要

Burn injury remains one of the most devastating burdens on global public health. The inflammation, caused by burn injury and transplantation of dermal scaffolds, often leads to delayed burn wound healing. Esculentoside A (EsA), with a strong anti-inflammatory capacity, is an available agent that might contribute to the treatment of burn wounds. However, the poor stability and toxicity of EsA limit its clinical application. In the present study, we constructed chitosan/alginate nanoparticles (EsA-CS/ALG-NPs) to improve sustainability and reduce toxicity followed by impregnation of the prepared EsA-CS/ALG-NPs into a collagen/chitosan scaffold (EsA-CS/ALG-NPs@CCS). The particle size, structural morphology, thermal properties, and chemical interaction of repaired nanoparticles were evaluated using the Nanometrics instrument, differential scanning calorimetry, transmission electron microscopy, and Fourier transform infrared spectroscopy, respectively. The hybrid EsA-CS/ALG-NPs@CCS was evaluated for physical characteristics, in vitro drug release, biocompatibility, and anti-inflammation capacity with RAW 264.7 cells and in vivo burn wound healing studies with SD rats. The results showed that we successfully constructed CS/ALG-NPs and optimized the preparation process to achieve the highest encapsulation efficiency. The hybrid EsA-CS/ALG-NPs@CCS, with reduced cytotoxicity and sustained release of EsA, could alleviate inflammation, decrease the ratio of M1 macrophages, and increase the proportion of M2 macrophages in vitro. It was demonstrated that 5 μg EsA CS/ALG-NPs@CCS not only reduces inflammatory cytokines secretion and inhibits M1 macrophages but also promotes the release of anti-inflammatory cytokines and activates M2 macrophages, thereby achieving accelerated and high-quality healing of burn wounds ultimately. In summary, our work suggests that the synergistic combination of EsA, nanoparticles, and scaffolds provided a promising strategy for treating burn injuries.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
拼搏一曲发布了新的文献求助10
1秒前
CAOHOU应助科研通管家采纳,获得10
2秒前
共享精神应助科研通管家采纳,获得10
2秒前
CAOHOU应助科研通管家采纳,获得10
2秒前
SYLH应助科研通管家采纳,获得20
2秒前
乐乐应助科研通管家采纳,获得10
2秒前
汉堡包应助科研通管家采纳,获得10
2秒前
FashionBoy应助科研通管家采纳,获得10
2秒前
桐桐应助科研通管家采纳,获得10
2秒前
2秒前
深情安青应助科研通管家采纳,获得10
2秒前
CAOHOU应助科研通管家采纳,获得10
2秒前
SYLH应助科研通管家采纳,获得10
2秒前
CAOHOU应助科研通管家采纳,获得10
2秒前
Owen应助科研通管家采纳,获得10
3秒前
SYLH应助科研通管家采纳,获得10
3秒前
SYLH应助科研通管家采纳,获得10
3秒前
3秒前
酷波er应助科研通管家采纳,获得10
3秒前
wushuwen发布了新的文献求助10
3秒前
4秒前
xuan完成签到,获得积分10
5秒前
完美世界应助段一帆采纳,获得10
7秒前
少敏敏完成签到,获得积分10
9秒前
may发布了新的文献求助10
9秒前
14秒前
16秒前
兜兜关注了科研通微信公众号
16秒前
wbh完成签到,获得积分10
17秒前
太牛的GGB发布了新的文献求助10
17秒前
wbh发布了新的文献求助10
19秒前
乐乐应助may采纳,获得10
19秒前
顺利的梦菲完成签到 ,获得积分10
20秒前
777完成签到 ,获得积分10
20秒前
上官若男应助忧郁盼夏采纳,获得10
21秒前
冷艳的姿发布了新的文献求助10
22秒前
量子星尘发布了新的文献求助10
24秒前
25秒前
27秒前
28秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989378
求助须知:如何正确求助?哪些是违规求助? 3531442
关于积分的说明 11254002
捐赠科研通 3270126
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173