枯草芽孢杆菌
同源重组
基因组
生物
遗传学
DNA
计算生物学
同源染色体
基因组工程
能力(人力资源)
染色体
细菌
基因
基因组编辑
社会心理学
心理学
作者
Wilfried J. J. Meijer,Andrés Miguel-Arribas
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 241-260
标识
DOI:10.1007/978-1-0716-3930-6_12
摘要
Bacillus subtilis is one of the best-studied bacteria and serves as a Gram-positive model system to address fundamental biological processes. Depending on conditions, a B. subtilis cell can initiate one out of various distinct differentiation processes to cope with changing environmental conditions. One of these differentiation processes is natural competence that allows cells to adsorb exogenous DNA and subsequently incorporate it into its chromosome by homologous recombination. Due to competence development, the genome of B. subtilis can be easily manipulated, and this has contributed to B. subtilis being a model system. In this chapter, we describe some of the most common genetic tools that can be used in combination with natural competence to tailor the genome of B. subtilis.
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