Preparation of Protein-like Silver–Cysteine Hybrid Nanowires and Application in Ultrasensitive Immunoassay of Cancer Biomarker

化学 免疫分析 检出限 半胱氨酸 水溶液 线性范围 纳米颗粒 色谱法 纳米技术 抗体 生物化学 有机化学 生物 材料科学 免疫学
作者
Wenjuan Chen,Liyan Zheng,Meilan Wang,Yuwu Chi,Guonan Chen
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:85 (20): 9655-9663 被引量:23
标识
DOI:10.1021/ac401961f
摘要

Novel protein-like silver–cysteine hybrid nanowires (p-SCNWs) have been synthesized by a green, simple, nontemplate, seedless, and one-step aqueous-phase approach. AgNO3 and l-cysteine were dissolved in distilled water, forming Ag–cysteine precipitates and HNO3. Under vigorous stirring, the pH of the solution was rapidly adjusted to 9.0 by addition of concentrated sodium hydroxide solution, leading to quick dissolution of the Ag-cysteine precipitates and sudden appearance of white precipitates of p-SCNWs. The p-SCNWs are monodispersed nanowires with diameter of 100 nm and length of tens of micrometers, and have abundant carboxyl (−COOH) and amine (−NH2) groups at their surfaces, large amounts of peptide-linkages and S-bonding silver ions (Ag+) inside, making them look and act like Ag-hybrid protein nanostructures. The abundant −COOH and −NH2 groups at the surfaces of p-SCNWs have been found to facilitate the reactions between the p-SCNWs and proteins including antibodies. Furthermore, the fact that the p-SCNWs contain large amounts of silver ions enables biofunctionalized p-SCNWs to be excellent signal amplifying chemiluminescence labels for ultrasensitive and highly selective detection of important antigens, such as cancer biomarkers. In this work, the immunoassay of carcinoembryonic antigen (CEA) in human serum was taken as an example to demonstrate the immunoassay applications of antibody-functionalized p-SCNWs. By the novel p-SCNW labels, CEA can be detected in the linear range from 5 to 400 fg/mL with a limit of detection (LOD) of 2.2 fg/mL (at signal-to-noise ratio of 3), which is much lower than that obtained by commercially available enzyme-linked immunosorbent assay (ELISA). Therefore, the synthesized p-SCNWs are envisioned to be an excellent carrier for proteins and related immunoassay strategy would have promising applications in ultrasensitive clinical screening of cancer biomarkers for early diagnostics of cancers.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
桐桐应助大炮台采纳,获得10
刚刚
qqa发布了新的文献求助10
1秒前
2秒前
共享精神应助xumengsuo采纳,获得10
2秒前
2秒前
hui发布了新的文献求助10
3秒前
夏青荷发布了新的文献求助10
3秒前
3秒前
4秒前
5秒前
wu发布了新的文献求助10
7秒前
7秒前
7秒前
Lucas应助地狱跳跳虎采纳,获得10
7秒前
8秒前
CrystalRmu完成签到,获得积分10
8秒前
9秒前
BIUBIU完成签到,获得积分10
9秒前
叁肆发布了新的文献求助10
10秒前
mokmok发布了新的文献求助10
10秒前
yy发布了新的文献求助30
11秒前
大炮台发布了新的文献求助10
11秒前
tansl1989发布了新的文献求助10
13秒前
13秒前
14秒前
范式完成签到,获得积分20
15秒前
15秒前
C2H5MgBr完成签到,获得积分10
16秒前
SciGPT应助loski采纳,获得10
17秒前
沉静的八宝粥完成签到,获得积分10
17秒前
人各有痣完成签到,获得积分10
17秒前
mokmok完成签到,获得积分10
18秒前
李爱国应助naonao采纳,获得10
19秒前
范式发布了新的文献求助10
20秒前
透明人完成签到,获得积分10
20秒前
tansl1989完成签到,获得积分10
21秒前
23秒前
LSX发布了新的文献求助10
23秒前
bkagyin应助火星上尔柳采纳,获得10
25秒前
25秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2500
Востребованный временем 2500
Aspects of Babylonian celestial divination : the lunar eclipse tablets of enuma anu enlil 1500
Agaricales of New Zealand 1: Pluteaceae - Entolomataceae 1040
Healthcare Finance: Modern Financial Analysis for Accelerating Biomedical Innovation 1000
Classics in Total Synthesis IV: New Targets, Strategies, Methods 1000
지식생태학: 생태학, 죽은 지식을 깨우다 600
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3459066
求助须知:如何正确求助?哪些是违规求助? 3053650
关于积分的说明 9037605
捐赠科研通 2742924
什么是DOI,文献DOI怎么找? 1504562
科研通“疑难数据库(出版商)”最低求助积分说明 695334
邀请新用户注册赠送积分活动 694589