半胱氨酸
荧光
谷胱甘肽
斯托克斯位移
斑马鱼
化学
生物物理学
吸收(声学)
生物活性
生物化学
生物
体外
材料科学
酶
物理
量子力学
复合材料
基因
作者
Rui Guo,Qing Liu,Yonghe Tang,Shushun Cai,Xiaoya Li,Xi Gong,Yingjie Yang,Weiying Lin
标识
DOI:10.1016/j.saa.2022.121230
摘要
The small molecule biological thiols, such as Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play crucial roles in maintaining various cellular vital activities. In the organism, abnormal levels of small-molecule biological thiols have been associated with a variety of diseases. Therefore, quantitative determination of biological thiols, especially Cys, is significant for understanding their functions in various biological processes. Thus, in this work we designed a new fluorescent probe Ty-Cys1 with a large Stokes shift of 207 nm to monitor Cysteine. The maximum absorption wavelength of Ty-Cys1 was 418 nm, and the maximum emission wavelength was 625 nm. Significantly, the novel probe Ty-Cys1 was effectively in detecting of Cys changes in living cells, zebrafishes, and living mice.
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