Electrochemical Tau Protein Immunosensor Based on MnS/GO/PANI and Magnetite‐incorporated Gold Nanoparticles

Abstract Herein, electrochemical tau protein immunosensor based on manganese sulfide nanoparticles/graphene oxide/polyaniline (MnS/GO/PANI) and magnetite‐incorporated gold nanoparticles (AuNPs@Fe 3 O 4 ) was constructed. After the modification of the glassy carbon electrode (GCE) with MnS/GO/PANI, the immobilization and conjugation of anti‐Tau capture antibody and antigen Tau protein were successfully completed on MnS/GO/PANI/GCE, respectively. Then, the conjugation of anti‐Tau secondary antibody was carried out to AuNPs@Fe 3 O 4 as signal amplification via amino‐gold affinity and the eventual immunosensor was accomplished by the distinctive interactions of electrode platform and signal amplification. Final immunosensor demonstrated the quantification limit (LOQ) of 1.0×10 −13 M and the detection limit (LOD) of 1.0×10 −14 M.