清脆的
生物
Cas9
小RNA
计算生物学
基因组编辑
非编码RNA
表观遗传学
核糖核酸
引导RNA
基因
遗传学
长非编码RNA
作者
Jie Yang,Xiaodan Meng,Jinchang Pan,Nan Jiang,Chengwei Zhou,Zhenhua Wu,Zhaohui Gong
出处
期刊:RNA Biology
[Taylor & Francis]
日期:2017-10-13
卷期号:15 (1): 35-43
被引量:89
标识
DOI:10.1080/15476286.2017.1391443
摘要
Cancer is characterized by multiple genetic and epigenetic alterations, including a higher prevalence of mutations of oncogenes and/or tumor suppressors. Mounting evidences have shown that noncoding RNAs (ncRNAs) are involved in the epigenetic regulation of cancer genes and their associated pathways. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) system, a revolutionary genome-editing technology, has shed light on ncRNA-based cancer therapy. Here, we briefly introduce the classifications and mechanisms of CRISPR/Cas9 system. Importantly, we mainly focused on the applications of CRISPR/Cas9 system as a molecular tool for ncRNA (microRNA, long noncoding RNA and circular RNA, etc.) editing in human cancers, and the novel techniques that are based on CRISPR/Cas9 system. Additionally, the off-target effects and the corresponding solutions as well as the challenges toward CRISPR/Cas9 were also evaluated and discussed. Long- and short-ncRNAs have been employed as targets in precision oncology, and CRISPR/Cas9-mediated ncRNA editing may provide an excellent way to cure cancer.
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