油红O
脂肪生成
脂肪酸合酶
脂肪生成
脂滴
内分泌学
脂肪细胞
内科学
脂肪组织
脂质代谢
化学
脂肪甘油三酯脂肪酶
3T3-L1
基因敲除
生物
脂解
生物化学
医学
细胞凋亡
作者
Jicui Chen,Hongtao Zhao,Xiaoli Ma,Yuchao Zhang,Sumei Lu,Yangang Wang,Chen Zong,Dandan Qin,Yuanmei Wang,Yingfeng Yang,Xiangdong Wang,Yuantao Liu
摘要
The aim of this study was to determine the direct role of liraglutide (LG) in adipogenesis and lipid metabolism.Lipid accumulation was evaluated by oil red O staining, quantitative real-time PCR (qPCR) was performed to determine glucagon-like peptide 1 receptor (GLP-1R), fatty acid synthase (FASN) and adipose triglyceride lipase (ATGL) expression in 3T3-L1 preadipocytes, differentiated adipocytes and in adipose tissues from mice. The effects of LG on 3T3-L1 adipogenesis and lipid metabolism were analyzed with qPCR, Western Blotting, oil red O staining, immunohistochemistry (IHC) and immunofluorescence (IF). All measurements were performed at least three times.LG increased the expression of differentiation marker genes and lipid accumulation during preadipocyte differentiation. In differentiated adipocytes, LG decreased FASN expression, and simultaneously led to CREB phosphorylation and ERK1/2 activation which were abolished by a GLP-1R antagonist, exendin (9-39). LG induced-FASN down-regulation was partially reversed by PKA and ERK1/2 inhibitors. Consistent with above in vitro findings, LG treatment significantly reduced FASN expression in visceral adipose tissues of ob/ob mice, and reduced body weight gain.LG promotes preadipocytes differentiation, and inhibits FASN expression in adipocytes. LG induced down-regulation of FASN is at least partially mediated by PKA and MAPK signaling pathways.
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