Effects of Hemp seed soft capsule on colonic ion transport in rats

AIMTo investigate the effect of Hemp seed soft capsule (HSCC) on colonic ion transport and its related mechanisms in constipation rats. METHODSSprague-Dawley male rats were randomly divided into three groups: normal group, constipation group and HSSC group.Rats in the constipation and HSSC groups were administrated loperamide 3 mg/kg per day orally for 12 d to induce the constipation model.Then, the HSSC group was given HSSC 0.126 g/kg per day by gavage for 7 d.The normal and constipation groups were treated with distilled water.After the treatment, the fecal wet weight and water content were measured.The basal short-circuit current (Isc ) and resistance were measured by an Ussing Chamber.Besides the in vivo drug delivery experiment above, an in vitro drug application experiment was also conducted.The accumulative concentrations of HSSC (0.1 mg/mL, 0.5 mg/mL, 1.0 mg/mL, 2.5 mg/mL, 5.0 mg/mL, 10.0 mg/mL and 25.0 mg/mL) were added to the normal isolated