EGF‐induced EGF‐receptor and MAP kinase phosphorylation in goat cumulus cells during in vitro maturation

生物 磷酸化 细胞生物学 体外 激酶 受体 生物化学
作者
Laurence Gall,Claire Boulesteix,Sylvie Ruffini,Guy Germain
出处
期刊:Molecular Reproduction and Development [Wiley]
卷期号:71 (4): 489-494 被引量:26
标识
DOI:10.1002/mrd.20317
摘要

Abstract EGF has been shown to influence meiotic maturation and development competence of oocyte in various mammalian species. We previously reported, in goat, that the EGF receptor (EGF‐R) was present both on cumulus cells and oocytes. Here, EGF‐induced signaling was investigated during the in vitro maturation process in goat cumulus–oocyte complexes (COCs). Cumulus cells and oocytes were subjected to Western immunoblotting analysis using anti‐MAP kinase, anti‐phosphotyrosine, anti‐phospho MAP kinase, and anti‐phospho EGF‐R antibodies. We demonstrated that treatment with EGF during the in vitro maturation process induced rapid tyrosine phosphorylation of EGF‐R in a time and concentration dependent manner in cumulus cells. A similar pattern of activation by phosphorylation was observed for MAP kinase upon EGF stimulation. AG 1478, an inhibitor of the EGF kinase, suppressed EGF‐stimulated phosphorylation of EGF‐R and also affected the MAP kinase activation. Treatment with the MEK inhibitor PD 98059 abolished EGF‐induced MAP kinase activation. We did not observe oocyte EGF‐R phosphorylation in our experiments during the in vitro maturation process. Our data indicate, in goat cumulus cells, that activation of EGF‐R by EGF triggers signaling through the MAP kinase pathway during in vitro maturation. This supports the hypothesis that the major site of action for EGF, that regulates oocyte maturation, is the cumulus cell. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
李爱国应助Godzilla采纳,获得10
刚刚
1秒前
1秒前
万能图书馆应助龙傲天采纳,获得10
1秒前
留胡子的书桃完成签到,获得积分10
1秒前
龙龙完成签到,获得积分10
2秒前
2秒前
吕洺旭发布了新的文献求助10
3秒前
66完成签到 ,获得积分10
4秒前
斯文败类应助chengying624采纳,获得10
4秒前
隐形曼青应助晨辉采纳,获得10
4秒前
4秒前
5秒前
拜拜驳回了reflux应助
5秒前
源老头完成签到,获得积分10
5秒前
Maevel发布了新的文献求助30
6秒前
L_完成签到 ,获得积分10
6秒前
6秒前
致行发布了新的文献求助10
6秒前
田様应助阵雨采纳,获得10
7秒前
小王完成签到,获得积分20
7秒前
小怀完成签到 ,获得积分10
7秒前
suan完成签到,获得积分10
8秒前
123123发布了新的文献求助10
8秒前
小二郎应助优雅的琳采纳,获得10
8秒前
rwewe完成签到,获得积分10
9秒前
9秒前
nannan发布了新的文献求助10
9秒前
锤死别人的锤完成签到,获得积分10
9秒前
遐蝶应助俞思含采纳,获得10
9秒前
10秒前
小王发布了新的文献求助10
10秒前
故意的梦之完成签到,获得积分10
10秒前
温柔的夜绿完成签到,获得积分10
10秒前
11秒前
11秒前
12秒前
耶耶完成签到,获得积分10
12秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Kelsen’s Legacy: Legal Normativity, International Law and Democracy 1000
Handbook on Inequality and Social Capital 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3546979
求助须知:如何正确求助?哪些是违规求助? 3123961
关于积分的说明 9357531
捐赠科研通 2822555
什么是DOI,文献DOI怎么找? 1551574
邀请新用户注册赠送积分活动 723561
科研通“疑难数据库(出版商)”最低求助积分说明 713801