Development of HPLC with UV‐VIS Detection for the Determination of the Level of Oxytetracycline in the Biological Matrix

Abstract An isocratic reversed‐phase high performance liquid chromatographic procedure was developed for the determination of oxytetracycline in the biological matrix. The procedure is based on isolation of the compound and the standard (oxytetracycline hydrochloride) from pig plasma using the Shimadzu C18 (500 mg) cartridge, with satisfactory recovery (92.50%) and specificity on a Varian ChromSep HPLC OmniSpher C18 (250×4.6 mm, 5 µm) column coupled with a UV‐VIS detector set at 360 nm. The suggested technique was shown to be linear (R2=0.9999) over the concentration range 25–500 ng/mL. These results clearly demonstrate that the HPLC method is a useful tool in many applications, particularly in veterinary medicine studies. Keywords: OxytetracyclineBiological matrixHPLC determinationUV‐VIS detectionMethod validation