Downregulation of kynureninase restrains cutaneous squamous cell carcinoma proliferation and represses the PI3K/AKT pathway

Clinical and Experimental DermatologyVolume 45, Issue 2 p. 194-201 Original Article Downregulation of kynureninase restrains cutaneous squamous cell carcinoma proliferation and represses the PI3K/AKT pathway C. Ci, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorC. Wu, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorD. Lyu, Corresponding Author lvdalun0776@163.com Department of, Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, China Correspondence: Dr Dalun Lyu, Department of Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, China E-mail: lvdalun0776@163.comSearch for more papers by this authorX. Chang, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorC. He, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorW. Liu, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorL. Chen, Department of, Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorW. Ding, Department of, Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this author C. Ci, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorC. Wu, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorD. Lyu, Corresponding Author lvdalun0776@163.com Department of, Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, China Correspondence: Dr Dalun Lyu, Department of Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, China E-mail: lvdalun0776@163.comSearch for more papers by this authorX. Chang, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorC. He, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorW. Liu, Department of, Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorL. Chen, Department of, Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this authorW. Ding, Department of, Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaSearch for more papers by this author First published: 16 August 2019 https://doi.org/10.1111/ced.14072Citations: 5 Conflict of interest: the authors declare that they have no conflicts of interest. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Summary Background The protein kynureninase (KYNU) has recently been reported to participate in the pathological processes of various diseases. Aim To explore the expression and the biological function of KYNU in cutaneous squamous cell carcinoma (cSCC). Methods Expression of KYNU in cSCC cell lines and tissues was firstly evaluated based on the Gene Expression Omnibus and the Oncomine databases. Quantitative reverse transcription–PCR was performed to determine the mRNA expression of KYNU in cSCC cell lines. Small interfering RNA (siRNA) was used for silencing KYNU. The effect of KYNU on the growth and motility of cSCC cells was determined by cell counting kit-8, wound-healing and Transwell assays, and western blotting was used to determine the protein expression of KYNU, AKT, phosphoinositide 3-kinase (PI3K), phosphorylated (p)-AKT and p-PI3K. Results KYNU was significantly upregulated in cSCC tissues and cell lines. Knockdown of KYNU using siRNA noticeably suppressed the proliferation, migration and invasion ability of SCL-1 cells (P < 0.01). Western blotting revealed that phosphorylation of AKT and PI3K was markedly inhibited after silencing KYNU. The ratios of p-AKT/AKT and p-PI3K/PI3K were significantly decreased in the si-KYNU group compared with the control group. Conclusion Depletion of KYNU could inhibit the growth of cSCC cells, possibly through modulating PI3K/AKT pathway. These data indicate that KYNU takes a key part in the malignant progression of cSCC, and could be considered as a promising therapeutic target for cSCC treatment. Citing Literature Volume45, Issue2March 2020Pages 194-201 RelatedInformation