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Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innate lymphoid cell‐dominant allergic airway inflammation through delivery of miR‐146a‐5p

先天性淋巴细胞 间充质干细胞 过敏性炎症 微泡 外体 免疫学 炎症 细胞生物学 免疫系统 生物 胞外囊泡 间质细胞 医学 先天免疫系统 癌症研究 小RNA 基因 生物化学
作者
Shu-Bin Fang,Hongyu Zhang,Cong Wang,Bi‐Xin He,Xiaoqing Liu,Xiang-Ci Meng,Yaqi Peng,Zhibin Xu,Xingliang Fan,Zhang-Jin Wu,Chen Dong,Lei Zheng,Song Guo Zheng,Qing‐Ling Fu
出处
期刊:Journal of extracellular vesicles [Wiley]
卷期号:9 (1) 被引量:139
标识
DOI:10.1080/20013078.2020.1723260
摘要

Group 2 innate lymphoid cells (ILC2s) are recently reported to play a more critical role in allergic diseases. We previously identified that mesenchymal stromal cells (MSCs) elicited therapeutic effects on allergic airway inflammation. Small extracellular vesicles (sEV) derived from MSCs possess striking advantages including low immunogenicity and high biosafety, and is extremely promising cell-free therapeutic agents. However, the effects of MSC-sEV on ILC2s are still unclear. Additionally, scalable isolation protocols are required for the mass production of homogenous MSC-sEV especially in clinical application. We previously reported that induced pluripotent stem cells-derived MSCs were the ideal cellular source for the large preparation of MSC-sEV. Here we developed a standardized scalable protocol of anion-exchange chromatography for isolation of MSC-sEV, and investigated the effects of MSC-sEV on ILC2 function from patients with allergic rhinitis and in a mouse ILC2-dominant asthma model. The characterization of MSC-sEV was successfully demonstrated in terms of size, morphology and specific markers. Using flow cytometry and human Cytokine Antibody Array, MSC-sEV but not fibroblasts-sEV (Fb-sEV) were found to significantly inhibit the function of human ILC2s. Similarly, systemic administration of MSC-sEV but not Fb-sEV exhibited an inhibition of ILC2 levels, inflammatory cell infiltration and mucus production in the lung, a reduction in levels of T helper 2 cytokines, and alleviation of airway hyperresponsiveness in a mouse model of asthma. Using RNA sequencing, miR-146a-5p was selected as the candidate to mediate the above effects of MSC-sEV. We next revealed the uptake of ILC2s to MSC-sEV, and that transfer of miR-146a-5p in MSC-sEV to ILC2s in part contributed to the effects of MSC-sEV on ILC2s in vitro and in a mouse model. In conclusion, we demonstrated that MSC-sEV were able to prevent ILC2-dominant allergic airway inflammation at least partially through miR-146a-5p, suggesting that MSC-sEV could be a novel cell-free strategy for the treatment of allergic diseases.
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