谷氨酰胺
沙眼衣原体
生物
谷氨酰胺分解
细胞内寄生虫
衣原体
谷氨酰胺酶
使负有责任或义务
衣原体
肽聚糖
细胞生物学
微生物学
类核
细胞内
细菌
病毒学
生物化学
遗传学
基因
氨基酸
大肠杆菌
生态学
作者
Karthika Rajeeve,Nadine Vollmuth,Sudha Janaki‐Raman,Thomas F. Wulff,Apoorva Baluapuri,Francesca R. Dejure,Claudia Huber,J. Stephen Fink,Maximilian Schmalhofer,W. Schmitz,Rajeeve Sivadasan,Martin Eilers,Elmar Wolf,Wolfgang Eisenreich,Almut Schulze,Jürgen Seibel,Thomas Rudel
出处
期刊:Nature microbiology
日期:2020-08-03
卷期号:5 (11): 1390-1402
被引量:31
标识
DOI:10.1038/s41564-020-0762-5
摘要
Obligate intracellular bacteria such as Chlamydia trachomatis undergo a complex developmental cycle between infectious, non-replicative elementary-body and non-infectious, replicative reticulate-body forms. Elementary bodies transform to reticulate bodies shortly after entering a host cell, a crucial process in infection, initiating chlamydial replication. As Chlamydia fail to replicate outside the host cell, it is unknown how the replicative part of the developmental cycle is initiated. Here we show, using a cell-free approach in axenic media, that the uptake of glutamine by the bacteria is crucial for peptidoglycan synthesis, which has a role in Chlamydia replication. The increased requirement for glutamine in infected cells is satisfied by reprogramming the glutamine metabolism in a c-Myc-dependent manner. Glutamine is effectively taken up by the glutamine transporter SLC1A5 and metabolized via glutaminase. Interference with this metabolic reprogramming limits the growth of Chlamydia. Intriguingly, Chlamydia failed to produce progeny in SLC1A5-knockout organoids and mice. Thus, we report on the central role of glutamine for the development of an obligate intracellular pathogenic bacterium and the reprogramming of host glutamine metabolism, which may provide a basis for innovative anti-infection strategies. This study describes the mechanism by which Chlamydia trachomatis reprogrammes host glutamine metabolism in a c-Myc-dependent manner. The authors show that glutamine uptake via the SLC15A transporter and glutaminolysis are crucial for peptidoglycan synthesis and Chlamydia replication.
科研通智能强力驱动
Strongly Powered by AbleSci AI