旁观者效应
辐照
化学
活性氧
缺氧(环境)
氧气
分子生物学
氧气增强率
自由基清除剂
细胞
癌症研究
细胞生物学
免疫学
生物
生物化学
氧化应激
物理
有机化学
核物理学
作者
Jianghong Zhang,K.M. Prise,Jin Yizun,Chunlin Shao
标识
DOI:10.3760/cma.j.issn.0254-5098.2009.01.003
摘要
Objective To investigate radiation induced bystander effect and its mechanism on hepatoma HepG2 cells under hypoxia condition. Methods Non-irradiated bystander hepatoma cells were co-cultured with irradiated cells or treated with the conditioned medium (CM) from irradiated cells, then micronuclei (MN) were measured for both irradiated cells and bystander cells. Results The MN yield of irradiated HepG2 cells under hypoxic condition was significantly lower than that under normoxia, the oxygen enhancement ratio of HepG2 cells of MN was 1.6. For both hypoxic and normoxic condition, the MN yield of bystander cells were obviously enhanced to a similar high level after co-culturing with irradiated cells or with CM treatment, and it also correlated with the irradiation dose. When the hypoxic HepG2 cells were treated with either DMSO, a scavenger of reactive oxygen species (ROS), or aminognanidine, an iNOS inhibitor, the yield of bystander MN was partly diminished, and the reducing rate of DMSO was 42.2%-46.7 %, the reducing rate of aminognanidine was 42 %. Conclusion ROS, NO and their downstream signal facets are involved in the radiation induced bystander effect of hypoxic HepG2 cells.
Key words:
Hypoxia; Hepatoma cell; Radiation-induced bystander effect; Micreuncleus; Signaling factor
科研通智能强力驱动
Strongly Powered by AbleSci AI