Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis

生物 Piwi相互作用RNA 微泡 表观遗传学 小RNA 后转座子 RNA干扰 DNA甲基化 遗传学 基因沉默 生殖系 计算生物学 细胞生物学 基因 核糖核酸 基因表达 转座因子 基因组
作者
Bo Zhang,Na Zhao,Lei Jia,Jinyuan Che,Xiaoxu He,Kefeng Liu,Baolong Bao
出处
期刊:BMC Genomics [BioMed Central]
卷期号:21 (1) 被引量:24
标识
DOI:10.1186/s12864-020-6660-7
摘要

Abstract Background Piwi-interacting RNAs (piRNAs) have been linked to epigenetic and post-transcriptional gene silencing of retrotransposons in germ line cells, particularly in spermatogenesis. Exosomes are important mediators of vesicle transport, and the piRNAs in exosomes might play an important role in cell communication and signal pathway regulation. Moreover, exosomic piRNAs are promising biomarkers for disease diagnosis and physiological status indication. We used Cynoglossus semilaevis because of its commercial value and its sexual dimorphism, particularly the sex reversed “pseudomales” who have a female karyotype, produce sperm, and copulate with normal females to produce viable offspring. Results To determine whether piRNAs from fish germ line cells have similar features, seminal plasma exosomes from half-smooth tongue sole, C. semilaevis , were identified, and their small RNAs were sequenced and analysed. We identified six signature piRNAs as biomarkers in exosomes of seminal plasma from males and pseudomale C. semilaevis . Bioinformatic analysis showed that all six signatures were sex-related, and four were DNA methylation-related and transposition-related piRNAs. Their expression profiles were verified using real-time quantitative PCR. The expression of the signature piRNAs was markedly higher in males than in pseudomales. The signature piRNAs could be exploited as male-specific biomarkers in this fish. Conclusions These signatures provide an effective tool to explore the regulatory mechanism of sex development in C. semilaevis and may provide guidance for future research on the function of piRNAs in the generative mechanism of sex reversed “pseudomales” in C. semilaevis .
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
1秒前
wahhhlt发布了新的文献求助10
1秒前
Mia完成签到,获得积分10
2秒前
XZY发布了新的文献求助10
3秒前
烟花应助roxy采纳,获得10
3秒前
热心市民小红花应助昵称采纳,获得10
4秒前
星辰大海应助fei采纳,获得10
4秒前
4秒前
顾越完成签到,获得积分10
5秒前
乐乐应助daxiong采纳,获得10
6秒前
pazhao发布了新的文献求助10
6秒前
13秒前
坦率的正豪完成签到,获得积分10
13秒前
酷波er应助甜美不评采纳,获得10
14秒前
15秒前
15秒前
木木发布了新的文献求助10
16秒前
Emily发布了新的文献求助10
16秒前
共行完成签到 ,获得积分10
16秒前
16秒前
顾矜应助科研通管家采纳,获得10
17秒前
FashionBoy应助科研通管家采纳,获得10
17秒前
ll应助科研通管家采纳,获得10
17秒前
科研通AI5应助科研通管家采纳,获得10
17秒前
ding应助科研通管家采纳,获得10
17秒前
汉堡包应助科研通管家采纳,获得10
17秒前
ll应助科研通管家采纳,获得10
18秒前
ll应助科研通管家采纳,获得10
18秒前
充电宝应助科研通管家采纳,获得10
18秒前
大模型应助科研通管家采纳,获得10
18秒前
星辰大海应助科研通管家采纳,获得10
18秒前
18秒前
18秒前
18秒前
19秒前
15858833895发布了新的文献求助10
20秒前
20秒前
在水一方应助影子采纳,获得10
20秒前
默默问晴完成签到,获得积分10
20秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975610
求助须知:如何正确求助?哪些是违规求助? 3519986
关于积分的说明 11200337
捐赠科研通 3256337
什么是DOI,文献DOI怎么找? 1798246
邀请新用户注册赠送积分活动 877446
科研通“疑难数据库(出版商)”最低求助积分说明 806357