Oxidative Stress and Antioxidants in Wheat Genotypes: Possible Mechanism of Water Stress Tolerance

Journal of Agronomy and Crop ScienceVolume 184, Issue 1 p. 55-61 Oxidative Stress and Antioxidants in Wheat Genotypes: Possible Mechanism of Water Stress Tolerance R. K. Sairam, R. K. Sairam Authors' address: Dr R. K. Sairam (corresponding author) and Dr D. C. Saxena, Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, IndiaSearch for more papers by this authorD. C. Saxena, D. C. Saxena Authors' address: Dr R. K. Sairam (corresponding author) and Dr D. C. Saxena, Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, IndiaSearch for more papers by this author R. K. Sairam, R. K. Sairam Authors' address: Dr R. K. Sairam (corresponding author) and Dr D. C. Saxena, Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, IndiaSearch for more papers by this authorD. C. Saxena, D. C. Saxena Authors' address: Dr R. K. Sairam (corresponding author) and Dr D. C. Saxena, Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, IndiaSearch for more papers by this author First published: 25 December 2001 https://doi.org/10.1046/j.1439-037x.2000.00358.xCitations: 178 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, India Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The role of plant antioxidant systems in water stress tolerance was studied in three contrasting wheat genotypes. Water stress imposed at different stages after anthesis resulted in an increase in lipid peroxidation and a decrease in membrane stability and chlorophyll and carotenoid contents. The antioxidant enzymes ascorbate peroxidase, glutathione reductase and non-specific peroxidase also increased significantly under water stress. Genotype PBW 175, which had highest ascorbate peroxidase, glutathione reductase and peroxidase activity, had the lowest lipid peroxidation (malondialdehyde content) and highest membrane stability and contents of chlorophyll and carotenoids under water stress, while the susceptible genotype WH 542 exhibited the lowest antioxidant enzyme activity, membrane stability and contents of chlorophyll and carotenoids and the highest lipid peroxidation. Genotype HD 2402 showed intermediate behaviour. It seems that drought tolerance of PBW 175, as represented by higher membrane stability and chlorophyll and carotenoid contents and lower lipid peroxidation, is related to its higher antioxidant enzyme activity. References Allen, R. D. 1995 Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol., 107, 1049 1054. Arnon, D. I. 1949 Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol., 24, 1 15. Baisak, R., D. Rana, P. B. B. Acharya, M. Kar 1994 Alteration in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiol., 35, 489 495. Cadenas, S. E. 1989 Biochemistry of oxygen toxicity. Ann. Rev. Biochem., 58, 79 110. Castillo, F. J., C. Penel, H. Greppin 1984 Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol., 74, 846 851. Davies, K. J. A. 1987 Protein damage and degradation by oxygen radicals. I. General aspects. J. Biol. Chem., 262, 9895 9901. Dhindsa, R. S., P. Plumb-Dhindsa, T. A. Thorne 1981 Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Expt. Bot., 32, 93 101. Egneus, H., U. Heber, M. Kirk 1975 Reduction of oxygen by the electron transport chain of chloroplasts during assimilation of carbon dioxide. Biochim. Biophys. Acta , 408, 252 268. Elstner, E. F. 1987 Metabolism of activated oxygen species. In: D. D. Davies, ed. The Biochemistry of Plants, Biochemistry of Metabolism, Vol. 11, pp. 253 315. Academic Press, San Diego, USA. Foster, J. G. & J. L. Hess 1982 Oxygen effects on maize leaf superoxide dismutase and glutathione reductase. Phytochemistry, 21, 1527 1532. Fridovich, I. 1986 Biological effects of superoxide radical. Arch. Biochem. Biophys., 247, 1 11. Gamble, P. E. & J. J. Burke 1984 Effect of water stress on the chloroplast antioxidant system. I. Alterations in glutathione reductase activity. Plant Physiol., 76, 615 621. Gillham, D. J. & A. D. Dodge 1986 Hydrogen peroxide-scavenging system with in pea chloroplasts. A quantitative study. Planta, 167, 246 251. Gillham, D. J. & A. D. Dodge 1987 Chloroplast superoxide and hydrogen peroxide scavenging systems from pea leaves: Seasonal variations. Plant Sci., 50, 105 109. Heath, R. L. & L. Packer 1968 Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125, 189 198. Imlay, J. A. & S. Linn 1988 DNA damage and oxygen radical toxicity. Science, 240, 1302 1309. Jagtap, V. & S. Bhargava 1995 Variation in the antioxidant metabolism of drought tolerant and drought susceptible varieties of Sorghum bicolor (L) Moench, exposed to high light, low water and high temperature stress. J. Plant Physiol., 145, 195 197. Knox, J. P. & A. D. Dodge 1985 Singlet oxygen and plants. Phytochemistry, 24, 889 896. Kraus, T. E., B. D. McKersie, R. A. Fletcher 1995 Paclobutrazol induced tolerance of wheat leaves to paraquat may involve increased antioxidant enzyme activity. J. Plant Physiol., 145, 570 576. Larson, R. A. 1988 The antioxidants of higher plants. Phytochemistry, 27, 969 978. Lichtenthaler, H. K. & W. R. Wellburn 1983 Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans., 11, 591 592. Liebler, D. C., D. S. Kling, D. J. Reed 1986 Antioxidant protection of phospholipid bilayers by α-tocopherol. Control of α-tocopherol status and lipid peroxidation by ascorbic acid and glutathione. J. Biol. Chem., 261, 12114 12119. Malan, C., M. M. Greyling, J. Gressel 1990 Correlation between Cu-Zn superoxide dismutase and glutathione reductase, and environmental and xenobiotic stress tolerance in maize inbreds. Plant Sci., 69, 157 166. Menconi, M., C. L. M. Sgherri, C. Pinzino, F. Navari-Izzo 1995 Activated oxygen production and detoxification in wheat plants subjected to a water deficit programme. J. Exp. Bot., 46, 1123 1130. Moran, J. F., M. Becana, I. Iturbe-Ormaetxe, S. Frenchilla, R. V. Aparicio, P. Tejo 1994 Drought induced oxidative stress in pea plants. Planta, 194, 346 352. Nakano, Y. & K. Asada 1981 Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 22, 867 880. Pastori, G. M. & V. S. Trippi 1992 Oxidative stress induces high rate of glutathione reductase synthesis in a drought resistant maize strain. Plant Cell Physiol., 33, 957 961. Premchandra, G. S., H. Saneoka, S. Ogata 1990 Cell membrane stability, an indicator of drought tolerance, as affected by applied nitrogen in soybean. J. Agric. Sci. Camb., 115, 63 66. Sairam, R. K. 1994 Effect of moisture stress on physiological activities of two contrasting wheat genotypes. Ind. J. Exp. Biol., 32, 594 597. Smith, I. K., T. L. Vierheller, C. A. Thorne 1988 Assay of glutathione reductase in crude tissue homogenates using, 5,5′-dithiobis (2-nitrobenzoic acid). Anal. Biochem., 175, 408 413. Turcsanyi, E., G. Suranyi, E. Lehoczki, G. Borbely 1994 Superoxide dismutase activity in response to paraquat resistance in Conyza canadensis (L.) Cronq. J. Plant Physiol., 144, 599 606. Van Rensburg, L. & G. H. J. Kruger 1994 Evaluation of components of oxidative stress metabolism for use in selection of drought tolerant cultivars of Nicotiana tobacum L. J. Plant Physiol., 143, 730 736. Walker, M. A. & B. D. McKersie 1993 Role of the ascorbate-glutathione antioxidant system in chilling resistance of tomato. J. Plant Physiol., 141, 234 239. Whetherley, P. E. 1950 Studies in the water relations of cotton plants. I. The field measurement of water deficit in leaves. New Phytol., 49, 81 87. Citing Literature Volume184, Issue1March 2000Pages 55-61 ReferencesRelatedInformation