生物
浮游植物
基因组
北极的
背景(考古学)
微生物群
生物地球化学循环
生态学
微生物生态学
海洋学
细菌
基因
营养物
遗传学
地质学
古生物学
作者
Anthony Duncan,Kerrie Barry,Chris Daum,Emiley A. Eloe‐Fadrosh,Simon Roux,Katrin Schmidt,Susannah G. Tringe,Klaus-Ulrich Valentin,Neha Varghese,Asaf Salamov,Igor V. Grigoriev,Richard M. Leggett,Vincent Moulton,Thomas Möck
出处
期刊:Microbiome
[Springer Nature]
日期:2022-04-28
卷期号:10 (1)
被引量:23
标识
DOI:10.1186/s40168-022-01254-7
摘要
Phytoplankton communities significantly contribute to global biogeochemical cycles of elements and underpin marine food webs. Although their uncultured genomic diversity has been estimated by planetary-scale metagenome sequencing and subsequent reconstruction of metagenome-assembled genomes (MAGs), this approach has yet to be applied for complex phytoplankton microbiomes from polar and non-polar oceans consisting of microbial eukaryotes and their associated prokaryotes.Here, we have assembled MAGs from chlorophyll a maximum layers in the surface of the Arctic and Atlantic Oceans enriched for species associations (microbiomes) with a focus on pico- and nanophytoplankton and their associated heterotrophic prokaryotes. From 679 Gbp and estimated 50 million genes in total, we recovered 143 MAGs of medium to high quality. Although there was a strict demarcation between Arctic and Atlantic MAGs, adjacent sampling stations in each ocean had 51-88% MAGs in common with most species associations between Prasinophytes and Proteobacteria. Phylogenetic placement revealed eukaryotic MAGs to be more diverse in the Arctic whereas prokaryotic MAGs were more diverse in the Atlantic Ocean. Approximately 70% of protein families were shared between Arctic and Atlantic MAGs for both prokaryotes and eukaryotes. However, eukaryotic MAGs had more protein families unique to the Arctic whereas prokaryotic MAGs had more families unique to the Atlantic.Our study provides a genomic context to complex phytoplankton microbiomes to reveal that their community structure was likely driven by significant differences in environmental conditions between the polar Arctic and warm surface waters of the tropical and subtropical Atlantic Ocean. Video Abstract.
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