Bioactive Alkaloids from the Beibu Gulf Coral-associated Fungus Acremonium sclerotigenum GXIMD 02501

The Beibu Gulf represents an underexplored reservoir of marine micro-/organisms and secondary metabolites.Three uncommon 4-hydroxy-2-pyridone alkaloids and one phenazine alkaloid were obtained from the Beibu Gulf coral-associated fungus Acremonium sclerotigenum GXIMD 02501 via OSMAC approach.They were identified as campyridones D (1) and A (2), ilicicolin H (3), and phenazine-1-carboxylic acid (4), respectively, by spectroscopic analysis and comparison with literature values.All of them were evaluated for cytotoxic, anti-Vibrio, and NF-κB luciferase inhibitory activities.Compounds 2 and 3 showed cytotoxicity against two prostate cancer cell lines, with IC50 values of 17.6 ± 1.3 and 5.5 ± 1.2 μM for PC-3, while 25.4 ± 1.7 and 11.9 ± 1.3 μM for 22Rv1, respectively.Besides, compound 4 showed promising anti-Vibrio activity with MIC values of 0.047-0.067mg/mL and also displayed inhibition of LPS-induced NF-κB activation at 10 μM.