检出限
化学
荧光
纳米团簇
猝灭(荧光)
分析化学(期刊)
纳米颗粒
色谱法
材料科学
胶体金
纳米技术
光学
物理
有机化学
作者
Jincheng Xiong,Shuang He,Zile Wang,Yuliang Xu,Liang Zhang,Huixia Zhang,Haiyang Jiang
标识
DOI:10.1016/j.jhazmat.2022.128316
摘要
Herein, a novel fluorescence quenching immunochromatographic test strip (FQICTS) for simultaneous detection of chloramphenicol (CAP) and amantadine (AMD) was developed on the basis of inner filter effect (IFE), with the combination of gold nanoparticles (AuNPs) and highly luminescent green-emitting gold nanoclusters (AuNCs) as the IFE quencher/donor pair. The AuNPs could quench the excitation light and emission light of AuNCs and achieve a high IFE efficiency due to dual spectral overlapping. Under optimal conditions, the “turn-on” mode of the AuNCs-based dual-readout FQICTS showed good linearity for CAP detection in chicken samples from 0.05 ng/g to 10 ng/g, with a limit of detection (LOD) of 0.043 ng/g. The linear range of AMD is 0.5–50 ng/g, with LOD of 0.45 ng/g. The visual LODs of CAP and AMD in “turn-on” mode were 200 and 10 times lower than that in “turn-off” mode, respectively. The “turn-on” mode of FQICTS showed high recovery for detecting CAP (82.5–94.5%) and AMD (81.9–110.7%) spiked into chicken samples. The performance and practicability of the established method were verified with commercial enzyme-immunoassay kits, and good correlations were observed. Overall, the newly developed AuNCs-based dual-readout FQICTS is a promising on-site screening tool for rapid, high-sensitivity detection of multiple food contaminants in practical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI